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Sialyl-Lewis^x on P-Selectin Glycoprotein Ligand-1 Is Regulated during Differentiation and Maturation of Dendritic Cells: A Mechanism Involving the Glycosyltransferases C2GnT1 and ST3Gal I¹

Sylvain Julien^*, Matthew J. Grimshaw^*, Mark Sutton-Smith, Julia Coleman^*, Howard R. Morris^,, Anne Dell, Joyce Taylor-Papadimitriou^* and Joy M. Burchell^2,*

^* Breast Cancer Biology Group, King’s College London, Guy’s Hospital, London, United Kingdom; Division of Molecular Biosciences, Imperial College, London, United Kingdom; and M-Scan, Wokingham, Berks, United Kingdom

To fulfil their function as APCs, dendritic cells (DC) and their precursors need to travel from blood to the peripheral tissues and, upon activation, migrate from tissues to draining lymph nodes. Because O-glycans play a role in T cell trafficking, we investigated the O-glycosylation profile of human monocyte-derived DC. Sialyl-Lewis^x (sLe^x), a glycan involved in extravasation via selectin binding, was found to be expressed exclusively on P-selectin glycoprotein ligand-1 in monocytes and immature DC. However, sLe^x was lost from mature DC even though these cells retained expression of P-selectin glycoprotein ligand-1. Maturation of DC led to a rapid change in the expression of glycosyltransferases involved in O-linked glycosylation. A down-regulation of C2GnT1 mRNA and enzymatic activity was observed with a concurrent up-regulation of ST3Gal I and ST6GalNAc II mRNA resulting in a loss of the core 2 structures required for sLe^x expression as a P-selectin ligand. Interestingly, the early regulation of these glycosyltransferases was mediated by PGE₂, which is known to be required for human DC migration. The pattern of O-glycosylation seen in mature cells was very similar to that expressed by naive T cells, which home to lymph nodes. Our data show that the regulation of O-glycosylation controls sLe^x expression, and also suggest that O-glycans may have a function in DC migration.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by Cancer Research U.K., the Biotechnology and Biological Sciences Research Council, and the Wellcome Trust. A.D. is a Biotechnology and Biological Sciences Research Council Professorial Fellow.

² Address correspondence and reprint requests to Dr. Joy M. Burchell, Breast Cancer Biology Group, Third Floor, Thomas Guy House, Guy’s Hospital, London, SE1 9RT, U.K. E-mail address: joy.burchell{at}kcl.ac.uk

³ Abbreviations used in this paper: DC, dendritic cell; sLe^x, sialyl-Lewis^x; PSGL-1, P-selectin glycoprotein ligand-1; MMP, matrix metalloprotease; rhu, recombinant human; BGN, 1-benzyl-2-acetamido-2-deoxy--D-galactopyranoside; qRT-PCR, quantitative RT-PCR; Ct, cycle threshold; MS, mass spectrometry; GlcNAc, N-acetylglucosamine.

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