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Unique Features and Distribution of the Chicken CD83⁺ Cell¹

Department of Veterinary Medicine, Centre for Veterinary Science, University of Cambridge, Cambridge, United Kingdom

The central importance of dendritic cells (DC) in both innate and acquired immunity is well recognized in the mammalian immune system. By contrast DC have yet to be characterized in avian species despite the fact that avian species such as the chicken have a well-developed immune system. CD83 has proven to be an excellent marker for DC in human and murine immune systems. In this study we identify chicken CD83 (chCD83) as the avian equivalent of the human and murine DC marker CD83. We demonstrate for the first time that unlike human and murine CD83, chCD83 is uniquely expressed in the B cell areas of secondary lymphoid organs and in organs with no human or murine equivalent such as the bursa and Harderian gland. Furthermore through multicolor immunofluorescence, we identify chCD83⁺ populations that have unique attributes akin to both DC and follicular DC. These attributes include colocalization with B cell microrenvironments, MHC class II expression, dendritic morphology, and distribution throughout peripheral and lymphoid tissues.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by funding of a studentship from the British Egg Marketing Board and by a research grant from the Biotechnology and Biological Sciences Research Council.

² Address correspondence and reprint requests to Dr. Chris Hansell, Chemokine Research Group, Division of Immunology, Infection and Inflammation, University of Glasgow, 120 University Place, Glasgow G12 8TA, Scotland; E-mail address: ch120j{at}clinmed.gla.ac.uk and address requests for chCD83 Abs to Professor Ian McConnell, Department of Veterinary Medicine, Centre for Veterinary Science, University of Cambridge, Madingley Road, Cambridge CB3 0ES, U.K.; E-mail address: im200{at}cam.ac.uk

³ Abbreviations used in this paper: DC, dendritic cell; chCD83, chicken CD83; FDC, follicular DC; PALS, periarteriolar lymphoid sheath; PELS, periellipsoid lymphoid sheath; MHC-II, MHC class II.