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* Division of Rheumatology B115, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045; and
Developmental Immunology, Pediatric Services, Massachusetts General Hospital, Boston, MA 02114
Immune complex-induced inflammation can be mediated by the classical pathway of complement. However, using mice genetically deficient in factor B or C4, we have shown that the collagen Ab-induced model of arthritis requires the alternative pathway of complement and is not dependent on the classical pathway. We now demonstrate that collagen Ab-induced arthritis is not altered in mice genetically deficient in either C1q or mannose-binding lectins A and C, or in both C1q and mannose-binding lectins. These in vivo results prove the ability of the alternative pathway to carry out pathologic complement activation in the combined absence of intact classical and lectin pathways. C3 activation was also examined in vitro by adherent collagen-anti-collagen immune complexes using sera from normal or complement-deficient mice. These results confirm the ability of the alternative pathway to mediate immune complex-induced C3 activation when C4 or C1q, or both C1q and mannose-binding lectins, are absent. However, when all three activation pathways of complement are intact, initiation by immune complexes occurs primarily by the classical pathway. These results indicate that the alternative pathway amplification loop, with its ability to greatly enhance C3 activation, is necessary to mediate inflammatory arthritis induced by adherent immune complexes.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by National Institutes of Health Grant AR51749.
2 Address correspondence and reprint requests to Dr. William P. Arend, Division of Rheumatology B115, University of Colorado at Denver and Health Sciences Center, Building M20, Room 3106, 1775 North Ursula St., Aurora, CO 80045. E-mail address: william.arend{at}uchsc.edu
3 Abbreviations used in this paper: RA, rheumatoid arthritis; adIC, adherent immune complexes; anti-CCP, Abs to cyclic citrullinated peptide; AP, alternative pathway; CII, type II collagen; CAIA, collagen Ab-induced arthritis; CIA, collagen-induced arthritis; CP, classical pathway; GPI, glucose-6-phosphate isomerase; IC, immune complexes; LP, lectin pathway; MBL, mannose-binding lectin; MASP, MBL-associated serine proteases; VSB, veronal saline buffer; WT, wild type.
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