| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Pathology, Jaharis Building, Tufts University School of Medicine, Boston, MA 02111;
Department of Biochemistry, Tufts University School of Medicine, Boston, MA 02111; and
Pediatrics and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605
Peripheral blood memory B cells latently infected with EBV bear somatic mutations and are typically isotype switched consistent with being classical Ag-selected memory B cells. In this work, we performed a comparative analysis of the expressed Ig genes between large sets of EBV-infected and uninfected peripheral blood B cells, isolated from the same infectious mononucleosis patients, to determine whether differences exist that could reveal the influence of EBV on the production and maintenance of these cells. We observed that EBV+ cells on average accumulated more somatic hypermutations than EBV– cells. In addition, they had more replacement mutations and a higher replacement-silent ratio of mutations in their CDRs. We also found that EBV occupies a skewed niche within the memory compartment, due to its exclusion from the CD27+IgD+IgM+ subset, but this skewing does not affect the overall structure of the compartment. These results indicate that EBV impacts the mutation and selection process of infected cells but that once they enter memory they cannot be distinguished from uninfected cells by host homeostasis mechanisms.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by United States Public Health Research Grants CA65883, AI18757, and AI062989 (to D.T.L.) and AI49320 (to K.L.).
2 Address correspondence and reprint requests to Dr. David A. Thorley-Lawson, Department of Pathology, Jaharis Building, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111. E-mail address: david.thorley-lawson{at}tufts.edu
3 Abbreviations used in this paper: CSR, class switch recombination; EBER, EBV-encoded RNA; FWR, framework region; GC, germinal center; IM, infectious mononucleosis; LMP, latent membrane protein; R, replacement; S, silent; SHM, somatic hypermutation; PB, peripheral blood.
This article has been cited by other articles:
|
|
 |
|
  S. Chaganti, E. M. Heath, W. Bergler, M. Kuo, M. Buettner, G. Niedobitek, A. B. Rickinson, and A. I. Bell Epstein-Barr virus colonization of tonsillar and peripheral blood B-cell subsets in primary infection and persistence Blood, June 18, 2009; 113(25): 6372 - 6381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Roughan and D. A. Thorley-Lawson The Intersection of Epstein-Barr Virus with the Germinal Center J. Virol., April 15, 2009; 83(8): 3968 - 3976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Al Tabaa, E. Tuaillon, K. Bollore, V. Foulongne, G. Petitjean, J.-M. Seigneurin, C. Duperray, C. Desgranges, and J.-P. Vendrell Functional Epstein-Barr virus reservoir in plasma cells derived from infected peripheral blood memory B cells Blood, January 15, 2009; 113(3): 604 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rovedo and R. Longnecker Epstein-Barr Virus Latent Membrane Protein 2A Preferentially Signals through the Src Family Kinase Lyn J. Virol., September 1, 2008; 82(17): 8520 - 8528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dorner, F. Zucol, C. Berger, R. Byland, G. T. Melroe, M. Bernasconi, R. F. Speck, and D. Nadal Distinct Ex Vivo Susceptibility of B-Cell Subsets to Epstein-Barr Virus Infection According to Differentiation Status and Tissue Origin J. Virol., May 1, 2008; 82(9): 4400 - 4412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Hadinoto, M. Shapiro, T. C. Greenough, J. L. Sullivan, K. Luzuriaga, and D. A. Thorley-Lawson On the dynamics of acute EBV infection and the pathogenesis of infectious mononucleosis Blood, February 1, 2008; 111(3): 1420 - 1427. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
