HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ordway, D. Articles by Basaraba, R. J. Search for Related Content PubMed PubMed Citation Articles by Ordway, D. Articles by Basaraba, R. J.

The Cellular Immune Response to Mycobacterium tuberculosis Infection in the Guinea Pig¹

Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523

Pulmonary tuberculosis in guinea pigs is an extremely useful model for drug and vaccine testing due to the fact that its pathological disease process is similar to that present in humans. Progress in this field has been hindered because the tools necessary to undertake a complete immunological analysis of the guinea pig cellular immune response against Mycobacterium tuberculosis have been lacking. In this study, we combined a new flow cytometric gating strategy with immunohistochemistry to track T cells, B cells, and the MIL4 Ab, which detects both guinea pig heterophils (neutrophils) and eosinophils, to provide the first documentation of the kinetics of influx and positioning of these cell populations. The results show that the responding T cells are mostly CD4 cells and that after day 30 of the infection numbers of these cells in the lungs drops dramatically. These appear to be replaced by a steady increase in B cells and granulocytes which was associated with worsening lung pathology. These data reveal new information about the cellular phenotypes which mediate protective immunity or host immunopathogenesis during M. tuberculosis infection in this key animal model.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by National Institutes of Health Grant AI-054697.

² Address correspondence and reprint requests to Dr. Diane Ordway, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682. E-mail address: D.Ordway-Rodriguez{at}colostate.edu

³ Abbreviations used in this paper: FSC, forward scatter; SSC, side scatter.

This article has been cited by other articles:

				A. Grover, J. Taylor, J. Troudt, A. Keyser, K. Arnett, L. Izzo, D. Rholl, and A. Izzo Kinetics of the Immune Response Profile in Guinea Pigs after Vaccination with Mycobacterium bovis BCG and Infection with Mycobacterium tuberculosis Infect. Immun., November 1, 2009; 77(11): 4837 - 4846. [Abstract] [Full Text] [PDF]

				J. C. Sung, L. Garcia-Contreras, J. L. VerBerkmoes, C. A. Peloquin, K. J. Elbert, A. J. Hickey, and D. A. Edwards Dry Powder Nitroimidazopyran Antibiotic PA-824 Aerosol for Inhalation Antimicrob. Agents Chemother., April 1, 2009; 53(4): 1338 - 1343. [Abstract] [Full Text] [PDF]

				A. Otsuka, I. Matsunaga, T. Komori, K. Tomita, Y. Toda, T. Manabe, Y. Miyachi, and M. Sugita Trehalose Dimycolate Elicits Eosinophilic Skin Hypersensitivity in Mycobacteria-Infected Guinea Pigs J. Immunol., December 15, 2008; 181(12): 8528 - 8533. [Abstract] [Full Text] [PDF]

				L. F. Shubitz, S. M. Dial, R. Perrill, R. Casement, and J. N. Galgiani Vaccine-Induced Cellular Immune Responses Differ from Innate Responses in Susceptible and Resistant Strains of Mice Infected with Coccidioides posadasii Infect. Immun., December 1, 2008; 76(12): 5553 - 5564. [Abstract] [Full Text] [PDF]

				D. R. Hoff, M. L. Caraway, E. J. Brooks, E. R. Driver, G. J. Ryan, C. A. Peloquin, I. M. Orme, R. J. Basaraba, and A. J. Lenaerts Metronidazole Lacks Antibacterial Activity in Guinea Pigs Infected with Mycobacterium tuberculosis Antimicrob. Agents Chemother., November 1, 2008; 52(11): 4137 - 4140. [Abstract] [Full Text] [PDF]

				A. S. Dharmadhikari and E. A. Nardell What Animal Models Teach Humans about Tuberculosis Am. J. Respir. Cell Mol. Biol., November 1, 2008; 39(5): 503 - 508. [Abstract] [Full Text] [PDF]

				D. Ordway, M. Henao-Tamayo, C. Shanley, E. E. Smith, G. Palanisamy, B. Wang, R. J. Basaraba, and I. M. Orme Influence of Mycobacterium bovis BCG Vaccination on Cellular Immune Response of Guinea Pigs Challenged with Mycobacterium tuberculosis Clin. Vaccine Immunol., August 1, 2008; 15(8): 1248 - 1258. [Abstract] [Full Text] [PDF]