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Cutting Edge: CD8⁺ T Cell Clones Possess the Potential to Differentiate into both High- and Low-Avidity Effector Cells¹

Department of Microbiology & Immunology, Wake Forest University School of Medicine, Winston-Salem, NC 27157

The property of functional avidity is recognized to be of critical importance in determining pathogen clearance. An unresolved question with regard to this property is whether distinct naive subsets exist that display inherent differences in their peptide sensitivity requirements for activation, i.e., functional avidity, or whether differences in peptide sensitivity are induced following peptide encounter. In this study, we demonstrate that naive populations that can give rise to both high- and low-avidity cells do not contain subsets that exhibit differences in the amount of peptide required for activation. Furthermore, we show that an individual T cell clone can generate both high- and low-avidity effectors. The work presented here provides the first formal demonstration that an individual cell can give rise to both high- and low-avidity progeny, suggesting that avidity modulation at the level of an individual cell may play an important role in the CD8⁺ T cell response generated in vivo.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by National Institutes of Health Grant R01 AI43591 (to M.A.A-M.). C.J.K was supported by a National Research Training Award Grant AI07401.

² Address correspondence and reprint requests to Dr. Martha A. Alexander-Miller, Department of Microbiology & Immunology, Room 5053, Hanes Building, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157. E-mail address: marthaam{at}wfubmc.edu

³ Abbreviations used in this paper: CTO, CellTracker Orange; CTG, CellTracker Green.

This article has been cited by other articles:

				C. J. Kroger, S. Amoah, and M. A. Alexander-Miller Cutting Edge: Dendritic Cells Prime a High Avidity CTL Response Independent of the Level of Presented Antigen J. Immunol., May 1, 2008; 180(9): 5784 - 5788. [Abstract] [Full Text] [PDF]