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Therapeutic Anti-Inflammatory Effects of Myeloid Cell Adenosine Receptor A2a Stimulation in Lipopolysaccharide-Induced Lung Injury¹

Jörg Reutershan^*,¶, Rebecca E. Cagnina^*,, Daniel Chang^*, Joel Linden^*, and Klaus Ley^2,*,,

^* Robert M. Berne Cardiovascular Research Center, Department of Medicine, Department of Biomedical Engineering, and Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908; and ^¶ Department of Anesthesiology and Intensive Care Medicine, University of Tübingen, Tübingen, Germany

To determine the role of the adenosine receptor A2a in a murine model of LPS-induced lung injury, migration of polymorphonuclear leukocytes (PMNs) into the different compartments of the lung was determined by flow cytometry, microvascular permeability was assessed by the extravasation of Evans blue, and the release of chemotactic cytokines into the alveolar airspace was determined by ELISA. Measurements were performed in wild-type and A2a gene-deficient mice (A2a^–/–). To differentiate the role of A2a on hemopoietic and nonhemopoietic cells, we created chimeric mice by transfer of bone marrow (BM) between wild-type and A2a^–/– mice and used mice that lacked A2a expression selectively on myeloid cells (A2a^flox/flox x LysM-cre). A specific A2a receptor agonist (ATL202) was used to evaluate its potential to reduce lung injury in vivo. In wild-type mice, therapeutic treatment with ATL202 reduced LPS-induced PMN recruitment, and release of cytokines. Pretreatment, but not posttreatment, also reduced Evans blue extravasation. In the BM chimeric mice lacking A2a on BM-derived cells, PMN migration into the alveolar space was increased by 50%. These findings were confirmed in A2a^flox/flox x LysM-cre mice. ATL202 was only effective when A2a was present on BM-derived cells. A2a agonists may be effective at curbing inflammatory lung tissue damage.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by German Research Foundation Grant RE 1683/2-1 (to J.R.) and by National Institutes of Health Grant HL73361 (to J.L.) (K.L., project 2).

² Address correspondence and reprint requests to Dr. Klaus Ley, Cardiovascular Research Center, University of Virginia Health System, P.O. Box 801394, Charlottesville, VA 22908-1394. E-mail address: klausley{at}virginia.edu

³ Abbreviations used in this paper: ARDS, acute respiratory distress syndrome; PMN, polymorphonuclear leukocyte; BAL, bronchoalveolar lavage fluid; BM, bone marrow.

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The JI 2007 179: 729-730. [Full Text]  

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