| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine-University, Dusseldorf, Germany
IFN-
orchestrates a potent antimicrobial host response. However, the underlying molecular basis for this immunological defense system is largely unknown. In a systematic approach to identify IFN--regulated host effector molecules, a notable number of transcripts with consensus GTP-binding motives were obtained. Further extensive transcriptome and genome analyses identified five novel family members of murine guanylate-binding proteins (mGBPs) now designated mGBP6, 7, 8, 9, and 10. Moreover, in this study, all 10 mGBP members (mGBP1–10) were extensively characterized. mGBPs are selectively up-regulated in vitro by a set of proinflammatory cytokines and TLR agonists as well as in vivo after Listeria monocytogenes and Toxoplasma gondii infection. After IFN- stimulation, mGBP1, 2, 3, 6, 7, and 9 are associated with intracellular Toxoplasma parasites and, interestingly, virulent Toxoplasma interfere with mGBP recruitment. Taken together, mGBPs comprise an important set of host defense molecules.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by Grants PF259/3-3, FOR729, and Leibniz (to K.P.) and GRK1045/1 (to S.B. and K.P.) from Deutsche Forschungsgemeinschaft and MUGEN (to K.P.).
2 D.D., C.K., and C.B.-G. contributed equally to this study.
3 Address correspondence and reprint requests to Dr. Sandra Beer or Dr. Klaus Pfeffer, Institute of Medical Microbiology and Hospital Hygiene, Universitaetsstrasse 1, Geb. 22.21, Dusseldorf, Germany. E-mail addresses: sandra.beer{at}uni-duesseldorf.de or klaus.pfeffer{at}uni-duesseldorf.de
4 Abbreviations used in this paper: GBP, guanylate-binding protein; IRG, immunity-related GTPase; PV, parasitophorous vacuole; VSV, vesicular stomatitis virus; IRG, immunity-related GTPase; hGBP, human GBP; mGBP, murine GBP; DAPI, 4',6-diamidino-2-phenylindole; iNOS, inducible NO synthase; MEF, murine embryonic fibroblast.
5 The online version of this article contains supplemental material.
This article has been cited by other articles:
|
|
 |
|
  G. Li, J. Zhang, Y. Sun, H. Wang, and Y. Wang The Evolutionarily Dynamic IFN-Inducible GTPase Proteins Play Conserved Immune Functions in Vertebrates and Cephalochordates Mol. Biol. Evol., July 1, 2009; 26(7): 1619 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Trilling, V. T. K. Le, A. Zimmermann, H. Ludwig, K. Pfeffer, G. Sutter, G. L. Smith, and H. Hengel Gamma Interferon-Induced Interferon Regulatory Factor 1-Dependent Antiviral Response Inhibits Vaccinia Virus Replication in Mouse but Not Human Fibroblasts J. Virol., April 15, 2009; 83(8): 3684 - 3695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Tokovenko, R. Golda, O. Protas, M. Obolenskaya, and A. El'skaya COTRASIF: conservation-aided transcription-factor-binding site finder Nucleic Acids Res., April 1, 2009; 37(7): e49 - e49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhao, D. J. P. Ferguson, D. C. Wilson, J. C. Howard, L. D. Sibley, and G. S. Yap Virulent Toxoplasma gondii Evade Immunity-Related GTPase-Mediated Parasite Vacuole Disruption within Primed Macrophages J. Immunol., March 15, 2009; 182(6): 3775 - 3781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Kim, S. Parikh, J. C. Sun, A. Myrick, L. L. Lanier, P. J. Rosenthal, and J. L. DeRisi Experimental Malaria Infection Triggers Early Expansion of Natural Killer Cells Infect. Immun., December 1, 2008; 76(12): 5873 - 5882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Weinlander, E. Naschberger, M. H. Lehmann, P. Tripal, W. Paster, H. Stockinger, C. Hohenadl, and M. Sturzl Guanylate binding protein-1 inhibits spreading and migration of endothelial cells through induction of integrin {alpha}4 expression FASEB J, December 1, 2008; 22(12): 4168 - 4178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Racz, X. Nadal, J. Alferink, J. E. Banos, J. Rehnelt, M. Martin, B. Pintado, A. Gutierrez-Adan, E. Sanguino, N. Bellora, et al. Interferon-{gamma} Is a Critical Modulator of CB2 Cannabinoid Receptor Signaling during Neuropathic Pain J. Neurosci., November 12, 2008; 28(46): 12136 - 12145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Pluvinet, R. Olivar, J. Krupinski, I. Herrero-Fresneda, A. Luque, J. Torras, J. M. Cruzado, J. M. Grinyo, L. Sumoy, and J. M. Aran CD40: an upstream master switch for endothelial cell activation uncovered by RNAi-coupled transcriptional profiling Blood, November 1, 2008; 112(9): 3624 - 3637. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
