HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hovav, A.-H. Articles by Letvin, N. L. Search for Related Content PubMed PubMed Citation Articles by Hovav, A.-H. Articles by Letvin, N. L.

Duration of Antigen Expression In Vivo following DNA Immunization Modifies the Magnitude, Contraction, and Secondary Responses of CD8⁺ T Lymphocytes¹

Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115

The duration of Ag expression in vivo has been reported to have a minimal impact on both the magnitude and kinetics of contraction of a pathogen-induced CD8⁺ T cell response. In this study, we controlled the duration of Ag expression by excising the ear pinnae following intradermal ear pinnae DNA immunization. This resulted in decreased magnitude, accelerated contraction and differentiation, and surprisingly greater secondary CD8⁺ T cell responses. Furthermore, we found delayed and prolonged Ag presentation in the immunized mice; however, this presentation was considerably decreased when the depot Ag was eliminated. These findings suggest that the magnitude and the contraction phase of the CD8⁺ T cell response following intradermal DNA immunization is regulated by the duration rather than the initial exposure to Ag.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by the National Institute of Allergy and Infectious Diseases Center for HIV/AIDS Vaccine Immunology Grant AI067854.

² Address correspondence and reprint requests to Dr. Norman L. Letvin, Department of Medicine, Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02115. E-mail address: nletvin{at}bidmc.harvard.edu

³ Abbreviations used in this paper: DC, dendritic cell; CD62L, CD62 ligand; LN, lymph node.

This article has been cited by other articles:

				J. D. Finn, J. Bassett, J. B. Millar, N. Grinshtein, T. C. Yang, R. Parsons, C. Evelegh, Y. Wan, R. J. Parks, and J. L. Bramson Persistence of Transgene Expression Influences CD8+ T-Cell Expansion and Maintenance following Immunization with Recombinant Adenovirus J. Virol., December 1, 2009; 83(23): 12027 - 12036. [Abstract] [Full Text] [PDF]

				M. Elnekave, M. Bivas-Benita, G. O. Gillard, P. Sircar, and A.-H. Hovav A Matter of Timing: Unsynchronized Antigen Expression and Antigen Presentation Diminish Secondary T Cell Responses J. Immunol., July 15, 2009; 183(2): 1013 - 1021. [Abstract] [Full Text] [PDF]

				A. A. Wilson, L. W. Kwok, A.-H. Hovav, S. J. Ohle, F. F. Little, A. Fine, and D. N. Kotton Sustained Expression of {alpha}1-Antitrypsin after Transplantation of Manipulated Hematopoietic Stem Cells Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 133 - 141. [Abstract] [Full Text] [PDF]