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Differentiation and Ig-Allele Switch in Cell Line WEHI-231¹

Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0414

Because of its susceptibility to apoptosis upon Ag receptor cross-linking and lack of IgD expression, cells of the mouse cell line WEHI-231 have been classified as immature B cells. In this study we show that early freezings of the WEHI-231 line express IgD but not CD93, which classifies the cells as more similar to mature B cells. Another, later line obviously has differentiated in culture and has all the hallmarks of activated B cells. But despite activation-induced cytidine deaminase expression, there is no switch in isotype; instead we found switching from one µ allele to the other. As a consequence of these findings, we now view the apoptosis studies in the WEHI-231 line to reflect properties of mature and activated B lymphocytes, respectively.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by Grant CA100266 from the National Institutes of Health.

² Address correspondence and reprint requests to Dr. Matthias Wabl, Department of Microbiology and Immunology, S-1075 Box 0414, University of California, 513 Parnassus Avenue, San Francisco, CA 94143-0670. E-mail address: mutator{at}ucsf.edu

³ Abbreviations used in this paper: AID, activation-induced cytidine deaminase; MCL, mantle cell lymphoma; NZB, New Zealand Black; V_P, productive VDJ; V_NP, nonproductive VDJ; CLL, chronic lymphocytic leukemia.