HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baatar, D. Articles by Biragyn, A. Search for Related Content PubMed PubMed Citation Articles by Baatar, D. Articles by Biragyn, A.

Human Peripheral Blood T Regulatory Cells (Tregs), Functionally Primed CCR4⁺ Tregs and Unprimed CCR4^– Tregs, Regulate Effector T Cells Using FasL¹

Dolgor Baatar^*, Purevdorj Olkhanud^*, Kenya Sumitomo^*, Dennis Taub^*, Ronald Gress and Arya Biragyn^2,*

^* Laboratory of Immunology, Gerontology Research Center, National Institute on Aging, Baltimore, MD 21224; and Experimental Transplantation and Immunology Branch, National Caner Institute, Bethesda, MD 20892

Regulatory CD25⁺CD4⁺ T cells (Tregs) play an important role in the control of peripheral tolerance. In this study we demonstrate that human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. The majority (75%) of freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs. Interestingly, CCR4^– Tregs require anti-CD3 Ab-mediated activation to acquire a regulatory activity, while CCR4⁺ Tregs appear to be already primed to suppress the proliferation of CD8⁺ T cells. CCR4 is also expressed on CD25^lowCD4⁺ T cells (CCR4⁺ non-Tregs) that mostly suppress Th1-type polarization without affecting T cell proliferation, presumably via the production of immunomodulatory cytokines like IL-10. In contrast, CCR4⁺ Tregs express FasL to primarily regulate T cell proliferation via a contact-mediated process involving FasL/Fas signaling, a major regulatory pathway of T cell homeostasis. Finally, we also demonstrate that the depletion of CCR4⁺ T cells leads to Th1-type polarization of CD4⁺ T cells and augmentation of CD8⁺ T cell responses to tumor Ags.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This research was supported by the Intramural Research Program of the National Institute on Aging, National Institutes of Health.

² Address correspondence and reprint requests to Dr. Arya Biragyn, Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Box 21, Baltimore, Maryland 21224. E-mail address: biragyna{at}mail.nih.gov

³ Abbreviations used in this paper: Treg, regulatory CD4⁺ T cell; CM, conditioned medium; DC, dendritic cell; FoxP3, Forkhead box P3; GZ, granzyme; nnTreg, natural naive Treg; PI, propidium iodide; TARC, thymus- and activation-regulated chemokine; Tr1, T regulatory type 1 (cell).

This article has been cited by other articles:

				H. Yano, T. Ishida, A. Inagaki, T. Ishii, J. Ding, S. Kusumoto, H. Komatsu, S. Iida, H. Inagaki, and R. Ueda Defucosylated Anti CC Chemokine Receptor 4 Monoclonal Antibody Combined with Immunomodulatory Cytokines: A Novel Immunotherapy for Aggressive/Refractory Mycosis Fungoides and Sezary Syndrome Clin. Cancer Res., November 1, 2007; 13(21): 6494 - 6500. [Abstract] [Full Text] [PDF]

				D. Baatar, P. Olkhanud, D. Newton, K. Sumitomo, and A. Biragyn CCR4-Expressing T Cell Tumors Can Be Specifically Controlled via Delivery of Toxins to Chemokine Receptors J. Immunol., August 1, 2007; 179(3): 1996 - 2004. [Abstract] [Full Text] [PDF]