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Cutting Edge: Recombinant Listeria monocytogenes Expressing a Single Immune-Dominant Peptide Confers Protective Immunity to Herpes Simplex Virus-1 Infection¹

Mark T. Orr^*, Nural N. Orgun^*, Christopher B. Wilson^*, and Sing Sing Way^2,

^* Department of Immunology and Department of Pediatrics, University of Washington School of Medicine, Seattle, WA 98195

The vast majority of the world’s population is infected with HSV. Although antiviral therapy can reduce the incidence of reactivation and asymptomatic viral shedding, and limit morbidity and mortality from active disease, it cannot cure infection. Therefore, the development of an effective vaccine is an important global health priority. In this study, we demonstrate that recombinant Listeria monocytogenes (Lm) expressing the H-2K^b glycoprotein B (gB)_498–505 peptide from HSV-1 triggers a robust CD8 T cell response to this Ag resulting in protective immunity to HSV infection. Following challenge with HSV-1, immune-competent mice primed with recombinant Lm-expressing gB_498–505 Ag were protected from HSV-induced paralysis. Protection was associated with dramatic reductions in recoverable virus, and early expansion of HSV-1-specific CD8 T cells in the regional lymph nodes. Thus, recombinant Lm-expressing Ag from HSV represents a promising new class of vaccines against HSV infection.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported in part by Puget Sound Partners for Global Health Pilot Project Award (to S.S.W.), an Infectious Disease Society of America Career Development Award (to S.S.W.), and National Institutes of Health Grants K08HD51584 (to S.S.W.) and R01HD018184 (to C.B.W.).

² Address correspondence and reprint requests to Dr. Sing Sing Way, Department of Pediatrics, University of Washington School of Medicine, 1959 Northeast Pacific Street, Box 357650, Seattle, WA 98195. E-mail address: singsing{at}u.washington.edu

³ Abbreviations used in this paper: gB, glycoprotein B; Lm, Listeria monocytogenes; HA, hemagglutinin.

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