HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Plitas, G. Articles by DeMatteo, R. P. Search for Related Content PubMed PubMed Citation Articles by Plitas, G. Articles by DeMatteo, R. P.

NK Dendritic Cells Are Innate Immune Responders to Listeria monocytogenes Infection¹

Hepatobiliary Service, Memorial Sloan-Kettering Cancer Center, New York, NY 10021

NK dendritic cells (NKDC) are recently described immunologic cells that possess both lytic and Ag-presenting function and produce prolific quantities of IFN-. The role of NKDC in innate immunity to bacterial infection is unknown. Because IFN- is important in the immune response to Listeria monocytogenes (LM), we hypothesized that NKDC play a critical role during LM infection in mice. We found that LM increased the frequency and activation state of NKDC in vivo. Using in vivo intracellular cytokine analysis, we demonstrated that NKDC are a major source of early IFN- during infection with LM. Adoptive transfer of wild-type NKDC into IFN--deficient recipients that were subsequently infected with LM decreased bacterial burden in the liver and spleen and prolonged survival. In contrast, NK cells were depleted early during LM infection, produced less IFN-, and conferred less protection upon adoptive transfer into IFN--deficient mice. In vitro, LM induction of IFN- secretion by NKDC depended on TLR9, in addition to IL-18 and IL-12. Our study establishes NKDC as innate immune responders to bacterial infection by virtue of their ability to secrete IFN-.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by National Institutes of Health Grants AI70658 and DK068346.

² Address correspondence and reprint requests to Dr. Ronald P. DeMatteo, Memorial Sloan-Kettering Cancer Center, Box 203, 1275 York Avenue, New York, NY 10021. E-mail address: dematter{at}mskcc.org

³ Abbreviations used in this paper: LM, Listeria monocytogenes; NKDC, NK dendritic cells; DC, dendritic cells; WT, wild type; hkLM, heat killed LM; ICC, intracellular cytokine; iCpG, inhibitory CpG.

This article has been cited by other articles:

				H. Hara, K. Tsuchiya, T. Nomura, I. Kawamura, S. Shoma, and M. Mitsuyama Dependency of Caspase-1 Activation Induced in Macrophages by Listeria monocytogenes on Cytolysin, Listeriolysin O, after Evasion from Phagosome into the Cytoplasm J. Immunol., June 15, 2008; 180(12): 7859 - 7868. [Abstract] [Full Text] [PDF]

				S. E. Warren, D. P. Mao, A. E. Rodriguez, E. A. Miao, and A. Aderem Multiple Nod-Like Receptors Activate Caspase 1 during Listeria monocytogenes Infection J. Immunol., June 1, 2008; 180(11): 7558 - 7564. [Abstract] [Full Text] [PDF]

				M. C. Siracusa, M. G. Overstreet, F. Housseau, A. L. Scott, and S. L. Klein 17{beta}-Estradiol Alters the Activity of Conventional and IFN-Producing Killer Dendritic Cells J. Immunol., February 1, 2008; 180(3): 1423 - 1431. [Abstract] [Full Text] [PDF]

				U. I. Chaudhry, G. Plitas, B. M. Burt, T. P. Kingham, J. R. Raab, and R. P. DeMatteo NK Dendritic Cells Expanded in IL-15 Exhibit Antitumor Responses In Vivo J. Immunol., October 1, 2007; 179(7): 4654 - 4660. [Abstract] [Full Text] [PDF]