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* Immunology Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia;
Department of Experimental Medicine, University of Melbourne, Parkville, Victoria, Australia; and
University of Western Sydney, Hawkesbury Campus, Richmond, New South Wales, Australia
The transcriptional repressor Blimp-1 (B lymphocyte-induced maturation protein 1) has been described as a "master regulator" of B cell differentiation into Ab-secreting cells (ASCs). Although there is mounting evidence for the importance and necessity of Blimp-1 in plasma cell development, there is uncertainty as to the role it plays in B cell differentiation of B cell subsets and the way in which it may interact with other transcription factors such as Pax5 and Bcl6 during ASC differentiation. Using a mouse expressing GFP under the control of the Blimp-1 regulatory elements (Blimp-1GFP/+), we examined the kinetics of Blimp-1 up-regulation in purified B cell subsets following activation. B1 cells showed the most rapid and pronounced up-regulation of Blimp-1 in response to the mitogens tested, followed by marginal zone B cells and then conventional B2 cells. Interestingly, only B1 cells substantially up-regulated Blimp-1 expression in response to CpG. B1 cells secreted negligible Ig upon isolation but were able to up-regulate Blimp-1 and initiate Ig secretion within 28 h of stimulation. Also of interest, B1 cells have a transcriptional factor profile that is intermediate between a naive B cell and an ASC, indicative of the semiactivated state of B1 cells. Transferred naive Blimp-1GFP/+ B1 and B2 cells both gave rise to ASCs in the bone marrow, suggesting no intrinsic barriers to B1 cell entry into the long-lived ASC compartment.
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1 This work was supported by the National Health and Medical Research Council of Australia. K.A.F. is the recipient of a Dora Lush Postgraduate Fellowship (National Health and Medical Research Council); N.D.H. is the recipient of a Victorian Cancer Council Fellowship; A.K. is the recipient of a Deutsche Forschungsgemeinschaft Fellowship; S.N. was supported by a Metcalf Fellowship (Walter and Eliza Hall Institute, Australia); and L.M.C. and D.M.T. are Senior Research Fellows of the National Health and Medical Research Council.
2 Address correspondence and reprint requests to Dr. David Tarlinton, Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, Australia. E-mail address: tarlinton{at}wehi.edu.au
3 Abbreviations used in this paper: MZ, marginal zone; ASC, Ab-secreting cell; Blimp-1, B lymphocyte-induced maturation protein 1; int, intermediate; IRF-4, IFN-regulatory factor 4; XBP-1, X box-binding protein 1.
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