HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Takenaka, S. Articles by Gauldie, J. Search for Related Content PubMed PubMed Citation Articles by Takenaka, S. Articles by Gauldie, J.

Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics¹

Center for Gene Therapeutics, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

Dendritic cells (DCs) residing in different tissues and exposed to different organisms are likely to have different reactivities to their surrounding environment. Many studies use in vitro generated DCs to examine functions of these cells, but such cells may not truly reflect the nature of DCs and their in situ activities in vivo. We have used magnetic label-based technique to isolate colonic DCs to conduct derailed characterization of these cells. Colonic DCs comprise mainly CD11b⁺ DCs with few CD8⁺ DCs or plasmacytoid DCs. Functionally, isolated colonic DCs are able to endocytose and process proteins, undergo maturation, and stimulate T cells to proliferate. Importantly, expression of TLRs by colonic DCs is significantly lower than that of their spleen counterparts; however, they appear to be as, or more, responsive to stimulation by oligodeoxynucleotides containing CpG motif based on their cytokine production. We speculate that colonic DCs have unique reactivities differing from DCs residing in other lymphoid tissues and are adapted for the unique microenvironment of the colonic mucosa and that these cells react uniquely to their environment.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by the Canadian Institute of Health Research.

² Address correspondence and reprint requests to Dr. Jack Gauldie, Centre for Gene Therapeutics, Department of Pathology and Molecular Medicine, MDCL-4017, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada. E-mail address: gauldie{at}mcmaster.ca

³ Abbreviations used in this paper: DC, dendritic cell; DAPI, 4',6'-diamidino-2-phenylindole; ODN, oligodeoxynucleotide; pDC, plasmacytoid DC.