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Department of Immunology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo, Japan
Recent reports have shown that IL-17-producing CD4+ T cells (Th17 cells) belong to a distinct helper T cell lineage and are critically involved in the pathogenesis of autoimmune diseases and allergies. However, the chemokine receptor profile of Th17 cells remains to be clarified. In this study, we report that human Th17 cells are identified as CCR2+CCR5– memory CD4+ T cells. Analysis of PBMC from healthy donors showed that CCR2+ cells produce much larger amounts of IL-17 than CCR2– cells, indicating the preferential expression of CCR2 on Th17 cells. Notably, CCR2+CCR5– memory CD4+ T cells produced a large amount of IL-17 and little IFN-
, whereas CCR2+CCR5+ cells reciprocally produced an enormous amount of IFN- but little IL-17. Moreover, a higher expression of T-bet was seen in the CCR5+ memory T cells. These results indicate that absence of CCR5 distinguishes human Th17 cells from Th1 cells.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by a Grant-in-Aid for Scientific Research from the Japan Society (S) to T.Y. and Grant-in-Aid for Young Scientists (Start-up) to T.A. for the Promotion of Science and Research Grants from the Ministry of Health, Labour and Welfare of Japan.
2 Address correspondence and reprint requests to Dr. Takashi Yamamura, Department of Immunology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan. E-mail address: yamamura{at}ncnp.go.jp
3 Abbreviations used in this paper: EAE, experimental autoimmune encephalomyelitis; DN, double negative; DP, double positive; SP, single positive; MFI, mean fluorescence intensity.
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