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Department of Pathobiology, University of Pennsylvania, Philadelphia, PA 19104
The generation of Th1 responses is important for resistance to intracellular pathogens, including the parasite, Leishmania major. Although IFN-
R/STAT1 signaling promotes a Th1 response via the up-regulation of T-bet, the requirement for STAT1 in Th1 cell differentiation remains controversial. Although in some cases Th1 cells develop independently of STAT1, STAT1–/– mice fail to develop a Th1 response during L. major infection. However, the interpretation of this result is complicated by the role STAT1 plays in Ag presentation and, more importantly, in elimination of parasites by macrophages, because both defective Ag presentation and increased parasite burden can influence Th cell development. To resolve this issue, we assessed the ability of STAT1–/– T cells to become Th1 cells and protect mice against L. major following adoptive transfer into STAT1-sufficient mice. We found that whereas T-bet is critical for the differentiation of protective Th1 cells during L. major infection, IFN-R and STAT1 are dispensable. Given that a STAT1-independent Th1 cell response was generated by STAT1-sufficient APCs, but not by STAT1–/– cells, we next addressed whether dendritic cells (DCs) require STAT1 signaling to effectively present Ag. We found that STAT1–/– DCs had impaired up-regulation of MHC and costimulatory molecules, and, as a consequence, the absence of STAT1 resulted in reduced Th1 cell priming. Taken together, these results demonstrate that T cell expression of STAT1 is not required for the development of Th1 cells protective against L. major and instead stress the importance of STAT1 signaling in DCs for the optimal induction of Th1 responses.
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1 This work was supported by National Institutes of Health Grant 35914 (to P.S.).
2 Address correspondence and reprint requests to Dr. Phillip Scott, Department of Pathobiology, School of Veterinary Medicine, 380 South University Avenue-Hill Pavilion 310B, Philadelphia, PA 19104. E-mail address: pscott{at}vet.upenn.edu
3 Abbreviations used in this paper: WT, wild type; BFA, brefeldin A; DC, dendritic cell; dhfr-ts–, dihydrofolate reductase-thymidylate synthase; dLN, draining lymph node; Leish-OVA, L. major parasites expressing OVA; LN, lymph node; SLA, soluble Leishmania Ag.
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