| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Institute of Immunology, Hannover Medical School, Hannover, Germany; and
Institute of Molecular Immunology, National Research Center, Munich, Germany
Dendritic cells (DC) are able to capture, process, and present exogenous Ag to CD8+ T lymphocytes through MHC class I, a process referred to as cross-presentation. In this study, we demonstrate that CD103+ (CD11chighCD11blow) and CD103– (CD11cintCD11bhigh) DC residing in the lung-draining bronchial lymph node (brLN) have evolved to acquire opposing functions in presenting innocuous inhaled Ag. Thus, under tolerogenic conditions, CD103– DC are specialized in presenting innocuous Ag to CD4+ T cells, whereas CD103+ DC, which do not express CD8
, are specialized in presenting Ag exclusively to CD8+ T cells. In CCR7-deficient but not in plt/plt mice, Ag-carrying CD103+ DC are largely absent in the brLN, although CD103+ DC are present in the lung of CCR7-deficient mice. As a consequence, adoptively transferred CD8+ T cells can be activated under tolerizing conditions in plt/plt but not in CCR7-deficient mice. These data reveal that CD103+ brLN DC are specialized in cross-presenting innocuous inhaled Ag in vivo. Because these cells are largely absent in CCR7–/– mice, our findings strongly suggest that brLN CD103+ DC are lung-derived and that expression of CCR7 is required for their migration from the lung into its draining lymph node.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by a Grant SFB587-B3 from the German Research Foundation (to R.F.).
2 Address correspondence and reprint requests to Dr. Reinhold Förster, Institute of Immunology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. E-mail address: foerster.reinhold{at}mh-hannover.de
3 Abbreviations used in this paper: DC, dendritic cell; BMDC, bone marrow-derived DC; LN, lymph node; brLN, bronchial LN; i.t., intratracheal(ly).
This article has been cited by other articles:
|
|
 |
|
  L. Fahlen-Yrlid, T. Gustafsson, J. Westlund, A. Holmberg, A. Strombeck, M. Blomquist, G. G. MacPherson, J. Holmgren, and U. Yrlid CD11chigh Dendritic Cells Are Essential for Activation of CD4+ T Cells and Generation of Specific Antibodies following Mucosal Immunization J. Immunol., October 15, 2009; 183(8): 5032 - 5041. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Lukens, D. Kruijsen, F. E. J. Coenjaerts, J. L. L. Kimpen, and G. M. van Bleek Respiratory Syncytial Virus-Induced Activation and Migration of Respiratory Dendritic Cells and Subsequent Antigen Presentation in the Lung-Draining Lymph Node J. Virol., July 15, 2009; 83(14): 7235 - 7243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Dunne, B. Moran, R. C. Cummins, and K. H. G. Mills CD11c+CD8{alpha}+ Dendritic Cells Promote Protective Immunity to Respiratory Infection with Bordetella pertussis J. Immunol., July 1, 2009; 183(1): 400 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Qiu, Y. Miyake, H. Kaise, H. Kitamura, O. Ohara, and M. Tanaka Novel Subset of CD8{alpha}+ Dendritic Cells Localized in the Marginal Zone Is Responsible for Tolerance to Cell-Associated Antigens J. Immunol., April 1, 2009; 182(7): 4127 - 4136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Guerrero-Plata, D. Kolli, C. Hong, A. Casola, and R. P. Garofalo Subversion of Pulmonary Dendritic Cell Function by Paramyxovirus Infections J. Immunol., March 1, 2009; 182(5): 3072 - 3083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Brewig, A. Kissenpfennig, B. Malissen, A. Veit, T. Bickert, B. Fleischer, S. Mostbock, and U. Ritter Priming of CD8+ and CD4+ T Cells in Experimental Leishmaniasis Is Initiated by Different Dendritic Cell Subtypes J. Immunol., January 15, 2009; 182(2): 774 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Jakubzick, M. Bogunovic, A. J. Bonito, E. L. Kuan, M. Merad, and G. J. Randolph Lymph-migrating, tissue-derived dendritic cells are minor constituents within steady-state lymph nodes J. Exp. Med., November 24, 2008; 205(12): 2839 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hildner, B. T. Edelson, W. E. Purtha, M. Diamond, H. Matsushita, M. Kohyama, B. Calderon, B. U. Schraml, E. R. Unanue, M. S. Diamond, et al. Batf3 Deficiency Reveals a Critical Role for CD8{alpha}+ Dendritic Cells in Cytotoxic T Cell Immunity Science, November 14, 2008; 322(5904): 1097 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-L. del Rio, J.-I. Rodriguez-Barbosa, J. Bolter, M. Ballmaier, O. Dittrich-Breiholz, M. Kracht, S. Jung, and R. Forster CX3CR1+c-kit+ Bone Marrow Cells Give Rise to CD103+ and CD103- Dendritic Cells with Distinct Functional Properties J. Immunol., November 1, 2008; 181(9): 6178 - 6188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jaensson, H. Uronen-Hansson, O. Pabst, B. Eksteen, J. Tian, J. L. Coombes, P.-L. Berg, T. Davidsson, F. Powrie, B. Johansson-Lindbom, et al. Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans J. Exp. Med., September 1, 2008; 205(9): 2139 - 2149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schlickum, H. Sennefelder, M. Friedrich, G. Harms, M. J. Lohse, P. Kilshaw, and M. P. Schon Integrin {alpha}E(CD103){beta}7 influences cellular shape and motility in a ligand-dependent fashion Blood, August 1, 2008; 112(3): 619 - 625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. GeurtsvanKessel, M. A.M. Willart, L. S. van Rijt, F. Muskens, M. Kool, C. Baas, K. Thielemans, C. Bennett, B. E. Clausen, H. C. Hoogsteden, et al. Clearance of influenza virus from the lung depends on migratory langerin+CD11b- but not plasmacytoid dendritic cells J. Exp. Med., July 7, 2008; 205(7): 1621 - 1634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. F. Lind, C. L. Ahonen, A. Wasiuk, Y. Kosaka, B. Becher, K. A. Bennett, and R. J. Noelle Dendritic Cells Require the NF-{kappa}B2 Pathway for Cross-Presentation of Soluble Antigens J. Immunol., July 1, 2008; 181(1): 354 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Hao, T. S. Kim, and T. J. Braciale Differential Response of Respiratory Dendritic Cell Subsets to Influenza Virus Infection J. Virol., May 15, 2008; 82(10): 4908 - 4919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kushwah, H. Cao, and J. Hu Characterization of Pulmonary T Cell Response to Helper-Dependent Adenoviral Vectors following Intranasal Delivery J. Immunol., March 15, 2008; 180(6): 4098 - 4108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jakubzick, F. Tacke, F. Ginhoux, A. J. Wagers, N. van Rooijen, M. Mack, M. Merad, and G. J. Randolph Blood Monocyte Subsets Differentially Give Rise to CD103+ and CD103- Pulmonary Dendritic Cell Populations J. Immunol., March 1, 2008; 180(5): 3019 - 3027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Lukacs-Kornek, S. Burgdorf, L. Diehl, S. Specht, M. Kornek, and C. Kurts The Kidney-Renal Lymph Node-System Contributes to Cross-Tolerance against Innocuous Circulating Antigen J. Immunol., January 15, 2008; 180(2): 706 - 715. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
