| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Division of Rheumatology and Immunology, Department of Medicine, Duke University, Durham, NC 27710; and
Medical Research Services, Durham Veterans Affairs Medical Center, Durham, NC 27705
High-mobility group protein 1 (HMGB1) is a nonhistone nuclear protein whose function depends on cellular location. Inside the cell, HMGB1 modulates a variety of important cellular processes, including transcription, whereas outside the cell, HMGB1 acts as a cytokine that can promote inflammation and mediate sepsis and arthritis in animal models. In in vitro studies, proinflammatory molecules such as LPS, lipoteichoic acid, polyinosinic-polycytidylic acid (poly(I:C)), TNF-
, and type I and II IFNs can induce HMGB1 release from macrophages. Although these agents can activate cells, they can also induce apoptosis under certain circumstances. Therefore, because of evidence that apoptotic as well as necrotic cells can contribute to HMGB1-mediated events in sepsis, we have investigated the relationship between apoptosis and HMGB1 release in macrophages and other cells. In these experiments, using RAW 264.7 cells as a model, LPS and poly(I:C) caused HMGB1 release into the medium whereas CpG ODN failed to induce this response. With both LPS and poly(I:C), the extent of HMGB1 release correlated with the occurrence of apoptosis as measured by caspase 3 activation, lactate dehydrogenase release, and TUNEL staining. Similar results were obtained with primary murine macrophages as well as human Jurkat T cells. For Jurkat cells, poly(I:C) and NO donors induced apoptosis as well as HMGB1 release. Together, these results indicate that HMGB1 release from macrophages is correlated with the occurrence of apoptosis and suggest that these processes reflect common mechanisms and can occur concomitantly.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by Veterans Affairs Medical Research Service, National Institutes of Health Grant AI44808, and a grant from the Lupus Research Institute.
2 Address correspondence and reprint requests to Dr. David S. Pisetsky, 151G Durham, Veterans Affairs Medical Center, 508 Fulton Street, Durham, NC 27705. E-mail address: piset001{at}mc.duke.edu
3 Abbreviations used in this paper: HMGB1, high-mobility group protein 1; poly(I:C), polyinosinic-polycytidylic acid; ODN, oligodeoxynucleotide; iNOS, inducible NO synthase; LDH, lactate dehydrogenase; PI, propidium iodide; TSA, trichostatin A; TRIF, Toll/IL-1R domain-containing adaptor-inducing IFN-
.
This article has been cited by other articles:
|
|
 |
|
  K. Kikuchi, K.-i. Kawahara, S. Tancharoen, F. Matsuda, Y. Morimoto, T. Ito, K. K. Biswas, K. Takenouchi, N. Miura, Y. Oyama, et al. The Free Radical Scavenger Edaravone Rescues Rats from Cerebral Infarction by Attenuating the Release of High-Mobility Group Box-1 in Neuronal Cells J. Pharmacol. Exp. Ther., June 1, 2009; 329(3): 865 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Kim, S.-J. Kim, I.-S. Lee, M.-S. Lee, S. Uematsu, S. Akira, and K. I. Oh Bacterial Endotoxin Induces the Release of High Mobility Group Box 1 via the IFN-{beta} Signaling Pathway J. Immunol., February 15, 2009; 182(4): 2458 - 2466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Aneja, A. Tsung, H. Sjodin, J. V. Gefter, R. L. Delude, T. R. Billiar, and M. P. Fink Preconditioning with high mobility group box 1 (HMGB1) induces lipopolysaccharide (LPS) tolerance J. Leukoc. Biol., November 1, 2008; 84(5): 1326 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Huston, H. Wang, M. Ochani, K. Ochani, M. Rosas-Ballina, M. Gallowitsch-Puerta, M. Ashok, L. Yang, K. J. Tracey, and H. Yang Splenectomy Protects against Sepsis Lethality and Reduces Serum HMGB1 Levels J. Immunol., September 1, 2008; 181(5): 3535 - 3539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, S. Tasaka, Y. Shiraishi, K. Fukunaga, W. Yamada, H. Seki, Y. Ogawa, K. Miyamoto, Y. Nakano, N. Hasegawa, et al. Role of Soluble Receptor for Advanced Glycation End Products on Endotoxin-induced Lung Injury Am. J. Respir. Crit. Care Med., August 15, 2008; 178(4): 356 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Han, J. Zhong, W. Wei, Y. Wang, Y. Huang, P. Yang, S. Purohit, Z. Dong, M.-H. Wang, J.-X. She, et al. Extracellular High-Mobility Group Box 1 Acts as an Innate Immune Mediator to Enhance Autoimmune Progression and Diabetes Onset in NOD Mice Diabetes, August 1, 2008; 57(8): 2118 - 2127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Kappes, J. Fahrer, M. S. Khodadoust, A. Tabbert, C. Strasser, N. Mor-Vaknin, M. Moreno-Villanueva, A. Burkle, D. M. Markovitz, and E. Ferrando-May DEK Is a Poly(ADP-Ribose) Acceptor in Apoptosis and Mediates Resistance to Genotoxic Stress Mol. Cell. Biol., May 15, 2008; 28(10): 3245 - 3257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W Jiang and D S Pisetsky Expression of high mobility group protein 1 in the sera of patients and mice with systemic lupus erythematosus Ann Rheum Dis, May 1, 2008; 67(5): 727 - 728. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
