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Harboring of Particulate Allergens within Secretory Compartments by Mast Cells following IgE/FcRI-Lipid Raft-Mediated Phagocytosis¹

Jeoung-Sook Shin^*, Christopher P. Shelburne^*, Cong Jin^*, E. Ann LeFurgey and Soman N. Abraham^2,*,,

^* Department of Pathology, Department of Cell Biology, Department of Molecular Genetics and Microbiology, and Department of Immunology, Duke University Medical Center, Durham, NC 27710

Although much is known regarding the exocytic responses of mast cells following allergen/IgE-mediated activation, little is currently known of the fate of the activating allergens, many of which are particles. We have found that IgE-bound particulate allergens were phagocytosed by activated mast cells in a lipid raft-dependent manner. The nascent allergen-containing phagosomes were found to transform into granule compartments by acquiring VAMP7 and serotonin and exhibited the capacity to empty their contents upon mast cell activation. When allergen-harboring mast cells were stimulated, the intracellular allergens were expelled intact and shown to activate adjacent mast cells. This capacity of mast cells to phagocytose and retain whole and antigenically intact allergens could potentially contribute to the course of inflammatory diseases such as asthma.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by National Institutes of Health Research Grants R01 AI35678, R37 DK50814, and R21 AI056101 and by a Senior Investigator Award from the Sandler Foundation for Asthma Research.

² Address correspondence and reprint requests to Dr. Soman N. Abraham, Department of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710. E-mail address: Soman.abraham{at}duke.edu

³ Abbreviations used in this paper: MC, mast cell; PSG, pollen starch granule; TNBS, 2,4,6-trinitrobenzenesulfonic acid; TNP, trinitrophenol; TRITC, tetramethylrhodamine isothiocyanate.

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