HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Butler, J. E. Articles by Wertz, N. Search for Related Content PubMed PubMed Citation Articles by Butler, J. E. Articles by Wertz, N.

Antibody Repertoire Development in Fetal and Neonatal Piglets. XVII. IgG Subclass Transcription Revisited with Emphasis on New IgG3¹

Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242

Fetal piglets offer an in vivo model for determining whether Ag-independent IgG subclass transcription proceeds in a manner that differs from subclass transcription in pigs exposed to environmental Ags and TLR ligands. Our data from 12,000 C clones from >60 piglets provide the first report on the relative usage of all known porcine C genes in fetal and young pigs. Studies revealed that among the six C genes, allelic variants of IgG1 comprised 50–80% of the repertoire, and IgG2 alleles comprised <10% in nearly all tissues. However, relative transcription of allelic variants of IgG1 randomly deviate from the 1:1 ratio expected in heterozygotes. Most surprising was the finding that IgG3 accounted for half of all C transcripts in the ileal Peyer’s patches (IPPs) and mesenteric lymph nodes but on average only 5% of the clones from the thymus, tonsil, spleen, peripheral blood, and bone marrow of newborns. Lymphoid tissues from late term fetuses revealed a similar expression pattern. Except for IgG3 in the IPPs and mesenteric lymph nodes, no stochastic pattern of C expression during development was seen in animals from mid-gestation through 5 mo. The age and tissue dependence of IgG3 transcription paralleled the developmental persistence of the IPP, and its near disappearance corresponds to the diversification of the preimmune VDJ repertoire in young piglets. We hypothesize that long-hinged porcine IgG3 may be important in preadaptive responses to T cell-independent Ags similar to those described for its murine namesake.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This research was supported by U.S. Department of Agriculture-Agricultural Research Service Cooperative Agreement 58-3625-4-155 and the University of Iowa Carver Trust.

² Address correspondence and reprint requests to Dr. John E. Butler, Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242. E-mail address: john-butler{at}uiowa.edu

³ Abbreviations used in this paper: CSR, class switch recombination; AID, activation-induced cytidine deaminase; BM, bone marrow: DG, day of gestations; IPP, ileal Peyer’s patch; JPP, jejunal Peyer’s patch; MAMP, microbe associated molecular pattern; MLN, mesenteric lymph node; MZ, marginal zone.

This article has been cited by other articles:

				J. E. Butler, N. Wertz, P. Weber, and K. M. Lager Porcine Reproductive and Respiratory Syndrome Virus Subverts Repertoire Development by Proliferation of Germline-Encoded B Cells of All Isotypes Bearing Hydrophobic Heavy Chain CDR3 J. Immunol., February 15, 2008; 180(4): 2347 - 2356. [Abstract] [Full Text] [PDF]

				J. E. Butler, C. D. Lemke, P. Weber, M. Sinkora, and K. M. Lager Antibody Repertoire Development in Fetal and Neonatal Piglets: XIX. Undiversified B Cells with Hydrophobic HCDR3s Preferentially Proliferate in the Porcine Reproductive and Respiratory Syndrome J. Immunol., May 15, 2007; 178(10): 6320 - 6331. [Abstract] [Full Text] [PDF]