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Stage-Specific Expression of Two Neighboring Crlz1 and IgJ Genes during B Cell Development Is Regulated by Their Chromatin Accessibility and Histone Acetylation¹

Jung-Hyun Lim^2,*, Sun-Jung Cho^2,*, Sung-Kyun Park^*, Jiyoung Kim^*, Daeho Cho, Wang Jae Lee and Chang-Joong Kang^3,*

^* Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin, Gyeonggi-do, Korea; Department of Biological Science, Sookmyung Women’s University, Seoul, Korea; and Department of Anatomy, College of Medicine, Seoul National University, Seoul, Korea

The IgJ gene is expressed in the plasma cell stage. However, its neighboring charged amino acid-rich leucine zipper 1 (Crlz1) gene, which is mapped 30 kb upstream of the IgJ gene in mice, is shown to be expressed in the pre-B cell stage. These stage-specific expressions of two neighboring genes are found to be regulated by their chromatin accessibility and acetylation. Hypersensitive site 1 on the IgJ promoter is opened in the plasma cells, whereas hypersensitive sites 9/10 on the Crlz1 promoter are opened in the pre-B cells. Furthermore, H3 and H4 histones toward the chromatin of the Crlz1 gene are found to be hyperacetylated, especially on H3, in the pre-B cells, whereas those toward the chromatin of the IgJ gene are found to be hyperacetylated in the plasma cells. Consistently, the hyperacetylation of H3 and H4 toward the chromatin of the IgJ gene but not the Crlz1 gene is induced by an IL-2 treatment of BCL1, which is a model cell line for studying the terminal differentiation of B cells.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, R05-2002-000739-0). This study was also supported by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (00-PJ1-PG3-21000-0016).

² J.-H.L. and S.-J.C. contributed equally to this work.

³ Address correspondence and reprint requests to Dr. Chang-Joong Kang, Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin, Gyeonggi-do 449-701, Korea. E-mail address: cjkang{at}khu.ac.kr

⁴ Abbreviations used in this paper: HSS, hypersensitive site; Crlz1, charged amino acid-rich leucine zipper 1; CBF, core-binding factor; ChIP, chromatin immunoprecipitation; EtBr, ethidium bromide; DRG, dorsal root ganglion.