HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Le Priol, Y. Articles by Combadière, B. Search for Related Content PubMed PubMed Citation Articles by Le Priol, Y. Articles by Combadière, B.

High Cytotoxic and Specific Migratory Potencies of Senescent CD8⁺CD57⁺ Cells in HIV-Infected and Uninfected Individuals¹

Yannick Le Priol^*, Denis Puthier, Cédric Lécureuil^*, Christophe Combadière^*, Patrice Debré^*, Catherine Nguyen and Béhazine Combadière^2,*

^* Institut National de la Santé et de la Recherche Médicale Unité 543, Université Pierre et Marie Curie Paris 6, Hôpital Pitié-Salpêtrière, Paris, France; and Institut National de la Santé et de la Recherche Médicale ERM206/TAGC, Université d’Aix-Marseille II, Parc Scientifique de Luminy, Marseille, France

CD8⁺CD57⁺ T lymphocytes, present at low levels in the peripheral blood of healthy individuals expand during HIV infection and remain elevated during chronic infection. Their role in the immune response remains unclear. We performed a large-scale gene array analysis (3158 genes) to characterize them and, interestingly, found no distinction in the transcriptional profiles of CD8⁺CD57⁺ T lymphocytes from HIV-infected and uninfected subjects. In both groups, these cells showed specificity for multiple Ags and produced large amounts of IFN- and TNF-. The transcriptional profiles of CD8⁺CD57⁺ and CD8⁺CD57^– cells, however, differed substantially. We propose that CD8⁺CD57⁺ cells were Ag-driven effector cells with very high cytotoxic effector potential including perforin, granzymes, and granulysin, regardless of HIV status. At both the messenger and protein levels, they expressed more adhesion molecules and fewer chemokine receptors (CCR7 and CXCR4) than CD8⁺CD57^– cells but expressed preferentially CX3CR1. The lower expression level of genes involved in cell cycle regulation showed limited proliferation capacities of CD8⁺CD57⁺ even in response to TCR and IL-2, IL-7, and IL-15 stimulation. CD8⁺CD57⁺ T cells from both HIV and uninfected subjects maintain effective cytotoxic potentials but are destined to migrate to nonlymphoid tissues without further cycling.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by Agence Nationale de Recherches sur le Syndrome d’immunodéficience acquise (SIDA) Y.L.P. was a recipient of a fellowship from the Agence Nationale de Recherche sur le SIDA.

² Address correspondence and reprint requests to Dr. Béhazine Combadière, Université Pierre et Marie Curie Paris 6, 91 bd de l’hôpital, 75013 Paris, France. E-mail address: combadie{at}ccr.jussieu.fr

³ Abbreviations used in this paper: UI, uninfected; FC, fold change; DC, dendritic cell; SNARF, seminaphthorhodafluor; p-c-jun, phosphorylated c-jun.

This article has been cited by other articles:

				C. Petrovas, B. Chaon, D. R. Ambrozak, D. A. Price, J. J. Melenhorst, B. J. Hill, C. Geldmacher, J. P. Casazza, P. K. Chattopadhyay, M. Roederer, et al. Differential Association of Programmed Death-1 and CD57 with Ex Vivo Survival of CD8+ T Cells in HIV Infection J. Immunol., July 15, 2009; 183(2): 1120 - 1132. [Abstract] [Full Text] [PDF]

				K. Ladell, M. K. Hellerstein, D. Cesar, R. Busch, D. Boban, and J. M. McCune Central Memory CD8+ T Cells Appear to Have a Shorter Lifespan and Reduced Abundance as a Function of HIV Disease Progression J. Immunol., June 15, 2008; 180(12): 7907 - 7918. [Abstract] [Full Text] [PDF]

				D. Verhoeven, S. Sankaran, M. Silvey, and S. Dandekar Antiviral Therapy during Primary Simian Immunodeficiency Virus Infection Fails To Prevent Acute Loss of CD4+ T Cells in Gut Mucosa but Enhances Their Rapid Restoration through Central Memory T Cells J. Virol., April 15, 2008; 82(8): 4016 - 4027. [Abstract] [Full Text] [PDF]

				V. Appay, A. Bosio, S. Lokan, Y. Wiencek, C. Biervert, D. Kusters, E. Devevre, D. Speiser, P. Romero, N. Rufer, et al. Sensitive Gene Expression Profiling of Human T Cell Subsets Reveals Parallel Post-Thymic Differentiation for CD4+ and CD8+ Lineages J. Immunol., December 1, 2007; 179(11): 7406 - 7414. [Abstract] [Full Text] [PDF]

				M. Pedroza-Seres, M. Linares, S. Voorduin, R.-R. Enrique, R. Lascurain, Y. Garfias, and M. C. Jimenez-Martinez Pars planitis is associated with an increased frequency of effector-memory CD57+ T cells Br J Ophthalmol, October 1, 2007; 91(10): 1393 - 1398. [Abstract] [Full Text] [PDF]

				D. Focosi and M. Petrini CD57 Expression on Lymphoma Microenvironment As a New Prognostic Marker Related to Immune Dysfunction J. Clin. Oncol., April 1, 2007; 25(10): 1289 - 1291. [Full Text] [PDF]