| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Héma-Québec, Recherche et Développement, Sainte-Foy, Quebec, Canada
In humans, up to 40% of peripheral B cells express CD27 and have hypermutated variable regions in their Ig genes. The CD27+ B cells are considered to be derived from germinal center following specific antigenic stimulation. Actually, somatic hypermutation in Ig genes and CD27 expression are hallmarks of memory B cells. However, the blood IgM+IgD+CD27+ B cells were recently associated to splenic marginal zone B cells and proposed to be a subset distinct from germinal center-derived memory B cells showing premutated Igs. The results presented herein further weaken this bona fide association because B cells expressing surface IgG, but not CD27, were found in human blood. Representing 1–4% of all peripheral B cells and 
25% of the IgG+ blood B cells, this population expressed mutated IgG genes showing antigenic selection characteristics but with lower mutation frequencies than that of CD27+IgG+ B cells. However, their morphology and phenotype were similar to that of CD27+IgG+ cells. Interestingly, the proportion of IgG2 over IgG3 transcripts was opposite in CD27–IgG+ and CD27+IgG+ cells, suggesting distinct functions or origins. Overall, these findings extend the memory B cell reservoir beyond the CD27+ compartment and could provide further insights into B cell disorders of unknown etiology.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 J.F.F. was supported by a Ph.D. fellowship from le Fond Québécois de la Recherche sur la Nature et les Technologies and from Héma-Québec.
2 Address correspondence and reprint requests to Dr. Sonia Néron, Héma-Québec, Recherche et Développement, 1009 route du Vallon, Sainte-Foy, Quebec G1V 5C3, Canada. E-mail address: sonia.neron{at}hema-quebec.qc.ca
3 Abbreviations used in this paper: ABC, ATP-binding cassette; MTG, MitoTracker Green; IMGT, ImMunoGeneTics; FR, framework region; R, replacement; S, silent; MFI, mean fluorescence intensity; FcRH4, FcR homolog 4.
This article has been cited by other articles:
|
|
 |
|
  G. R.A. Ehrhardt, A. Hijikata, H. Kitamura, O. Ohara, J.-Y. Wang, and M. D. Cooper Discriminating gene expression profiles of memory B cell subpopulations J. Exp. Med., July 14, 2008; (2008) jem.20072682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Moir, J. Ho, A. Malaspina, W. Wang, A. C. DiPoto, M. A. O'Shea, G. Roby, S. Kottilil, J. Arthos, M. A. Proschan, et al. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals J. Exp. Med., July 14, 2008; (2008) jem.20072683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Weller, M. Mamani-Matsuda, C. Picard, C. Cordier, D. Lecoeuche, F. Gauthier, J.-C. Weill, and C.-A. Reynaud Somatic diversification in the absence of antigen-driven responses is the hallmark of the IgM+IgD+CD27+ B cell repertoire in infants J. Exp. Med., June 9, 2008; 205(6): 1331 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Forconi, E. Sozzi, D. Rossi, S. S. Sahota, T. Amato, D. Raspadori, L. Trentin, L. Leoncini, G. Gaidano, and F. Lauria Selective influences in the expressed immunoglobulin heavy and light chain gene repertoire in hairy cell leukemia Haematologica, May 1, 2008; 93(5): 697 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Tangye and K. L. Good Human IgM+CD27+ B Cells: Memory B Cells or "Memory" B Cells? J. Immunol., July 1, 2007; 179(1): 13 - 19. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
