|
|
||||||||
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110
Type I IFN-producing cells (IPC) are sentinels of viral infections. Identification and functional characterization of these cells have been difficult because of their small numbers in blood and tissues and their complex cell surface phenotype. To overcome this problem in mice, mAbs recognizing IPC-specific cell surface molecules have been generated. In this study, we report the identification of new Abs specific for mouse IPC, which recognize the bone marrow stromal cell Ag 2 (BST2). Interestingly, previously reported IPC-specific Abs 120G8 and plasmacytoid dendritic cell Ag-1 also recognize BST2. BST2 is predominantly specific for mouse IPC in naive mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-
. The activation-induced promiscuous expression of BST2 described in this study has important implications for the use of anti-BST2 Abs in identification and depletion of IPC. Finally, we show that BST2 resides within an intracellular compartment corresponding to the Golgi apparatus, and may be involved in trafficking secreted cytokines in IPC.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 M.C. is supported by National Institutes of Health Grant R01CA109 673-01A1, and A.L.B. by Infectious Diseases Training Grant 2T32A10717226.
2 Address correspondence and reprint requests to Dr. Marco Colonna, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110. E-mail address: mcolonna{at}pathology.wustl.edu
3 Abbreviations used in this paper: IPC, type I IFN-producing cell; BST2, bone marrow stromal cell Ag 2; CTx, cholera toxin; DC, dendritic cell; FLT3L, fms-like tyrosine kinase 3 ligand; IFNAR, IFN-
receptor; IFNGR, IFN-
receptor; PDCA1, plasmacytoid DC Ag-1.
This article has been cited by other articles:
![]() |
W. Cao, L. Bover, M. Cho, X. Wen, S. Hanabuchi, M. Bao, D. B. Rosen, Y.-H. Wang, J. L. Shaw, Q. Du, et al. Regulation of TLR7/9 responses in plasmacytoid dendritic cells by BST2 and ILT7 receptor interaction J. Exp. Med., July 6, 2009; 206(7): 1603 - 1614. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Cheon and G. R. Stark Unphosphorylated STAT1 prolongs the expression of interferon-induced immune regulatory genes PNAS, June 9, 2009; 106(23): 9373 - 9378. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Masuyama, T. Kuronita, R. Tanaka, T. Muto, Y. Hirota, A. Takigawa, H. Fujita, Y. Aso, J. Amano, and Y. Tanaka HM1.24 Is Internalized from Lipid Rafts by Clathrin-mediated Endocytosis through Interaction with {alpha}-Adaptin J. Biol. Chem., June 5, 2009; 284(23): 15927 - 15941. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Capini, M. Jaturanpinyo, H.-I Chang, S. Mutalik, A. McNally, S. Street, R. Steptoe, B. O'Sullivan, N. Davies, and R. Thomas Antigen-Specific Suppression of Inflammatory Arthritis Using Liposomes J. Immunol., March 15, 2009; 182(6): 3556 - 3565. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Rollason, V. Korolchuk, C. Hamilton, M. Jepson, and G. Banting A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells J. Cell Biol., March 9, 2009; 184(5): 721 - 736. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Sakuma, T. Noda, S. Urata, Y. Kawaoka, and J. Yasuda Inhibition of Lassa and Marburg Virus Production by Tetherin J. Virol., March 1, 2009; 83(5): 2382 - 2385. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Kamimura, H. Iwai, J. Piao, M. Hashiguchi, and M. Azuma The Glucocorticoid-Induced TNF Receptor-Related Protein (GITR)-GITR Ligand Pathway Acts As a Mediator of Cutaneous Dendritic Cell Migration and Promotes T Cell-Mediated Acquired Immunity J. Immunol., March 1, 2009; 182(5): 2708 - 2716. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Zhu, M. M. Augustine, T. Azuma, L. Luo, S. Yao, S. Anand, A. C. Rietz, J. Huang, H. Xu, A. S. Flies, et al. B7-H4-deficient mice display augmented neutrophil-mediated innate immunity Blood, February 19, 2009; 113(8): 1759 - 1767. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Jouvenet, S. J. D. Neil, M. Zhadina, T. Zang, Z. Kratovac, Y. Lee, M. McNatt, T. Hatziioannou, and P. D. Bieniasz Broad-Spectrum Inhibition of Retroviral and Filoviral Particle Release by Tetherin J. Virol., February 15, 2009; 83(4): 1837 - 1844. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Banovic, K. A. Markey, R. D. Kuns, S. D. Olver, N. C. Raffelt, A. L. Don, M. A. Degli-Esposti, C. R. Engwerda, K. P. A. MacDonald, and G. R. Hill Graft-versus-Host Disease Prevents the Maturation of Plasmacytoid Dendritic Cells J. Immunol., January 15, 2009; 182(2): 912 - 920. [Abstract] [Full Text] [PDF] |
||||
![]() |
L.-H. Tai, M.-L. Goulet, S. Belanger, N. Toyama-Sorimachi, N. Fodil-Cornu, S. M. Vidal, A. D. Troke, D. W. McVicar, and A. P. Makrigiannis Positive regulation of plasmacytoid dendritic cell function via Ly49Q recognition of class I MHC J. Exp. Med., December 22, 2008; 205(13): 3187 - 3199. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Mouries, G. Moron, G. Schlecht, N. Escriou, G. Dadaglio, and C. Leclerc Plasmacytoid dendritic cells efficiently cross-prime naive T cells in vivo after TLR activation Blood, November 1, 2008; 112(9): 3713 - 3722. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Bochtler, A. Kroger, R. Schirmbeck, and J. Reimann Type I IFN-Induced, NKT Cell-Mediated Negative Control of CD8 T Cell Priming by Dendritic Cells J. Immunol., August 1, 2008; 181(3): 1633 - 1643. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. H. GeurtsvanKessel, M. A.M. Willart, L. S. van Rijt, F. Muskens, M. Kool, C. Baas, K. Thielemans, C. Bennett, B. E. Clausen, H. C. Hoogsteden, et al. Clearance of influenza virus from the lung depends on migratory langerin+CD11b- but not plasmacytoid dendritic cells J. Exp. Med., July 7, 2008; 205(7): 1621 - 1634. [Abstract] [Full Text] [PDF] |
||||
![]() |
A.-C. Brehin, J. Mouries, M.-P. Frenkiel, G. Dadaglio, P. Despres, M. Lafon, and T. Couderc Dynamics of Immune Cell Recruitment during West Nile Encephalitis and Identification of a New CD19+B220-BST-2+ Leukocyte Population J. Immunol., May 15, 2008; 180(10): 6760 - 6767. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Y. Lee, J. S. Weinstein, D. C. Nacionales, P. O. Scumpia, Y. Li, E. Butfiloski, N. van Rooijen, L. Moldawer, M. Satoh, and W. H. Reeves A Novel Type I IFN-Producing Cell Subset in Murine Lupus J. Immunol., April 1, 2008; 180(7): 5101 - 5108. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Watarai, E. Sekine, S. Inoue, R. Nakagawa, T. Kaisho, and M. Taniguchi PDC-TREM, a plasmacytoid dendritic cell-specific receptor, is responsible for augmented production of type I interferon PNAS, February 26, 2008; 105(8): 2993 - 2998. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Zucchini, G. Bessou, S. H. Robbins, L. Chasson, A. Raper, P. R. Crocker, and M. Dalod Individual plasmacytoid dendritic cells are major contributors to the production of multiple innate cytokines in an organ-specific manner during viral infection Int. Immunol., January 1, 2008; 20(1): 45 - 56. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. L. Blasius, W. Barchet, M. Cella, and M. Colonna Development and function of murine B220+CD11c+NK1.1+ cells identify them as a subset of NK cells J. Exp. Med., October 29, 2007; 204(11): 2561 - 2568. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Sapoznikov, J. A.A. Fischer, T. Zaft, R. Krauthgamer, A. Dzionek, and S. Jung Organ-dependent in vivo priming of naive CD4+,but not CD8+,T cells by plasmacytoid dendritic cells J. Exp. Med., August 6, 2007; 204(8): 1923 - 1933. [Abstract] [Full Text] [PDF] |
||||
![]() |
H.-J. Wu, H. Sawaya, B. Binstadt, M. Brickelmaier, A. Blasius, L. Gorelik, U. Mahmood, R. Weissleder, J. Carulli, C. Benoist, et al. Inflammatory arthritis can be reined in by CpG-induced DC NK cell cross talk J. Exp. Med., August 6, 2007; 204(8): 1911 - 1922. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |