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Medical Research Council Centre for Immune Regulation, Institute for Biomedical Research, Birmingham Medical School, Birmingham, United Kingdom
We report here the quantitative expression of a set of immunity-related genes, including TNF family members, chemokine receptors, and transcription factors, in a CD4+CD3– accessory cell. By correlating gene expression between cell-sorted populations of defined phenotype, we show that the genetic fingerprint of these CD4+CD3– cells is distinct from dendritic cells, plasmacytoid dendritic cells, T cells, B cells, and NK cells. In contrast, it is highly similar to CD4+CD3– cells isolated from embryonic and neonatal tissues, with the exception that only adult populations express OX40L and CD30L. We have previously reported that IL-7 signals regulate CD30L expression. In the present study, we show that both neonatal and adult CD4+CD3– cells express the TNF family member, death receptor 3 (TNFRSF25), and that addition of TL1A (TNFSF15), the ligand for death receptor 3, up-regulates OX40L on neonatal CD4+CD3– cells. Finally, we demonstrate that this differentiation occurs in vivo: neonatal CD4+CD3– cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 This work was supported by a Wellcome Programme Grant (to P.J.L.L.).
2 Address correspondence and reprint requests to Dr. Peter J. L. Lane, Medical Research Council Centre for Immune Regulation, Institute for Biomedical Research, Birmingham Medical School, Birmingham B15 2TT, U.K. E-mail address: p.j.l.lane{at}bham.ac.uk
3 Abbreviations used in this paper: GC, germinal center; 
2m, 2-microglobulin; CC, correlation coefficient; DC, dendritic cell; DR3, death receptor 3; 
c, common -chain; HVEM, herpes virus entry mediator; LT, lymphotoxin; LIGHT, LT-related inducible ligand that competes for glycoprotein D binding to HVEM on T cell; pDC, plasmacytoid DC; TRANCE, TNF-related activation-induced cytokine; RANK, receptor activator of NF-
B.
4 The online version of this article contains supplemental material.
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