HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wolniak, K. L. Articles by Waldschmidt, T. J. Search for Related Content PubMed PubMed Citation Articles by Wolniak, K. L. Articles by Waldschmidt, T. J. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB NITROPHENOLS

Characterization of (4-Hydroxy-3-Nitrophenyl)Acetyl (NP)-Specific Germinal Center B Cells and Antigen-Binding B220^– Cells after Primary NP Challenge in Mice¹

Kristy L. Wolniak^*,, Randolph J. Noelle and Thomas J. Waldschmidt^2,*,

^* Department of Pathology and Interdisciplinary Graduate Program in Immunology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242; and Department of Microbiology, Dartmouth Medical School, Lebanon, NH 03755

Previous studies examining the primary germinal center (GC) response to SRBC in mice demonstrated a steady ratio of IgM⁺ to isotype-switched GC B cells and a persistent population of GC B cells with a founder phenotype. These characteristics held true at the inductive, plateau, and dissociative phases of the GC response, suggesting a steady-state environment. To test whether these characteristics apply to the primary response of other T cell-dependent Ags, the present study examined the GC response after challenge with (4-hydroxy-3-nitrophenyl)acetyl (NP) in C57BL/6 mice. Multiparameter flow cytometric analysis was used to assess the phenotype of splenic NP-reactive cells at multiple time points after immunization. Results of these studies demonstrated the characteristics of the SRBC-induced GC reaction to be fully maintained in the NP response. In particular, there was a steady ratio of nonswitched to switched B cells, with the majority of NP-reactive GC B cells displaying IgM. In addition, a substantial frequency of B220^– NP-binding cells was observed in the spleen at later time points after NP challenge. Although these cells were IgE⁺, they were found to express both and L chains and display the high-affinity IgE Fc (FcRI) receptor, suggesting that this population is not of B cell origin. Adoptive transfer studies further demonstrated the B220^– NP-binding subset to be derived from the myeloid lineage.

This article has been cited by other articles:

				L. Quemeneur, V. Angeli, M. Chopin, and R. Jessberger SWAP-70 deficiency causes high-affinity plasma cell generation despite impaired germinal center formation Blood, March 1, 2008; 111(5): 2714 - 2724. [Abstract] [Full Text] [PDF]

				K. Fink, N. Manjarrez-Orduno, A. Schildknecht, J. Weber, B. M. Senn, R. M. Zinkernagel, and H. Hengartner B Cell Activation State-Governed Formation of Germinal Centers following Viral Infection J. Immunol., November 1, 2007; 179(9): 5877 - 5885. [Abstract] [Full Text] [PDF]

				B. de Andres, I. Cortegano, N. Serrano, B. del Rio, P. Martin, P. Gonzalo, M. A. R. Marcos, and M. L. Gaspar A Population of CD19highCD45R /lowCD21low B Lymphocytes Poised for Spontaneous Secretion of IgG and IgA Antibodies J. Immunol., October 15, 2007; 179(8): 5326 - 5334. [Abstract] [Full Text] [PDF]