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Antigen-Specific CD8⁺ T Cells Respond to Chlamydia trachomatis in the Genital Mucosa¹

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115

Following sexual transmission, Chlamydia trachomatis specifically targets genital tract epithelial cells. Because epithelial cells are readily recognized by CD8⁺ T cells, the response of CD8⁺ T cells to Chlamydia infection has been explored in a number of studies. It has been shown that CD8⁺ T cells are present in the genital tracts of mice following C. trachomatis infection, but the specificity of these T cells has remained undefined. To determine whether Chlamydia-specific CD8⁺ T cells migrate to the genital tract in response to Chlamydia infection, we generated retrogenic mice that express a TCR specific for a Chlamydia-specific T cell Ag CrpA. T cells from the retrogenic mice were transferred into naive recipient animals to increase the frequency of Chlamydia-specific T cells to a level at which they could be tracked during primary infection. We observed that the Chlamydia-specific retrogenic T cells proliferated in lymph nodes draining the genital tract in response to genital infection with C. trachomatis. Furthermore, we found that these cells acquired the ability to produce IFN- and migrated into the genital mucosa of the infected mice.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by Grants AI039558 and AI055900 from the National Institute of Allergy and Infectious Diseases.

² Address correspondence and reprint requests to Dr. Michael N. Starnbach, Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115. E-mail address: starnbach{at}hms.harvard.edu

³ Abbreviations used in this paper: DLN, draining lymph node; BMM, bone marrow-derived macrophage; IFU, inclusion forming unit.

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