HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bilenki, L. Articles by Yang, X. Search for Related Content PubMed PubMed Citation Articles by Bilenki, L. Articles by Yang, X.

Adoptive Transfer of CD8⁺ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8^– DC

Laboratory for Infection and Immunity, Departments of Immunology and Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada

Chlamydial infections are serious public health concerns worldwide. In this study, we examined the role of dendritic cell (DC) subsets in inducing protective immunity against chlamydial infection using an adoptive transfer approach. We found that CD11c⁺CD8⁺ (double-positive, DP) DC, compared with CD11c⁺CD8^– (single-positive, SP) DC isolated from infected mice, are more potent inducers of protective immunity. Specifically, mice pretreated with DPDC from infected mice, upon infection with Chlamydia trachomatis mouse pneumonitis (MoPn), experienced significantly less severe body weight loss and in vivo chlamydial growth. Analysis of MoPn-driven cytokine production by immune cells revealed that mice that were treated with DPDC produced significantly higher levels of Th1 (TNF-, IFN-, and IL-12) but lower levels of Th2 (IL-4, IL-5, and IL-13)-related cytokines than the recipients of SPDC following infection challenge. Moreover, DPDC-treated mice displayed significantly higher levels of MoPn-specific IgG2a production and delayed-type hypersensitivity responses compared with SPDC-treated mice. Furthermore, DPDC isolated from infected mice produced higher amounts of IL-12 and IL-10 in vitro in comparison with SPDC. These data indicate that CD8⁺ DC have a significantly higher capacity in inducing protective immunity compared with CD8^– DC, demonstrating the crucial role of DC1-like cells in eliciting protection against C. trachomatis infection

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Address correspondence and reprint requests to Dr. Xi Yang, Laboratory for Infection and Immunity, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Room 523, 730 William Avenue, Winnipeg, Manitoba R3E 0W3, Canada. E-mail address: yangxi{at}cc.umanitoba.ca

² Abbreviations used in this paper MoPn, Chlamydia trachomatis mouse pneumonitis strain; DC, dendritic cell; DPDC, CD11c⁺CD8⁺ DC; SPDC, CD11c⁺CD8^– DC; EB, elementary body; DTH, delayed-type hypersensitivity; iDPDC, infected DPDC; IFU, inclusion-forming unit; iSPDC, infected SPDC; KO, knockout; MOMP, major outer membrane protein; nDPDC, naive DPDC; nSPDC, naive SPDC; SPG, sucrose-phosphate-glutamic acid.

This article has been cited by other articles:

				H. Bai, J. Cheng, X. Gao, A. G. Joyee, Y. Fan, S. Wang, L. Jiao, Z. Yao, and X. Yang IL-17/Th17 Promotes Type 1 T Cell Immunity against Pulmonary Intracellular Bacterial Infection through Modulating Dendritic Cell Function J. Immunol., November 1, 2009; 183(9): 5886 - 5895. [Abstract] [Full Text] [PDF]

				X. Jiang, C. Shen, J. Rey-Ladino, H. Yu, and R. C. Brunham Characterization of Murine Dendritic Cell Line JAWS II and Primary Bone Marrow-Derived Dendritic Cells in Chlamydia muridarum Antigen Presentation and Induction of Protective Immunity Infect. Immun., June 1, 2008; 76(6): 2392 - 2401. [Abstract] [Full Text] [PDF]

				X. Han, Y. Fan, S. Wang, L. Jiao, H. Qiu, and X. Yang NK Cells Contribute to Intracellular Bacterial Infection-Mediated Inhibition of Allergic Responses J. Immunol., April 1, 2008; 180(7): 4621 - 4628. [Abstract] [Full Text] [PDF]

				E. Kim, S. H. Kim, S. Kim, D. Cho, and T. S. Kim AIMP1/p43 Protein Induces the Maturation of Bone Marrow-Derived Dendritic Cells with T Helper Type 1-Polarizing Ability J. Immunol., March 1, 2008; 180(5): 2894 - 2902. [Abstract] [Full Text] [PDF]

				F. G. Gao, D. F. Wan, and J. R. Gu Ex vivo Nicotine Stimulation Augments the Efficacy of Therapeutic Bone Marrow-Derived Dendritic Cell Vaccination Clin. Cancer Res., June 15, 2007; 13(12): 3706 - 3712. [Abstract] [Full Text] [PDF]