HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Card, J. W. Articles by Zeldin, D. C. Search for Related Content PubMed PubMed Citation Articles by Card, J. W. Articles by Zeldin, D. C.

Gender Differences in Murine Airway Responsiveness and Lipopolysaccharide-Induced Inflammation¹

Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709

The roles of gender and sex hormones in lung function and disease are complex and not completely understood. The present study examined the influence of gender on lung function and respiratory mechanics in naive mice and on acute airway inflammation and hyperresponsiveness induced by intratracheal LPS administration. Basal lung function characteristics did not differ between naive males and females, but males demonstrated significantly greater airway responsiveness than females following aerosolized methacholine challenge as evidenced by increased respiratory system resistance and elastance (p < 0.05). Following LPS administration, males developed more severe hypothermia and greater airway hyperresponsiveness than females (p < 0.05). Inflammatory indices including bronchoalveolar lavage fluid total cells, neutrophils, and TNF- content were greater in males than in females 6 h following LPS administration (p < 0.05), whereas whole-lung TLR-4 protein levels did not differ among treatment groups, suggesting that differential expression of TLR-4 before or after LPS exposure did not underlie the observed inflammatory outcomes. Gonadectomy decreased airway inflammation in males but did not alter inflammation in females, whereas administration of exogenous testosterone to intact females increased their inflammatory responses to levels observed in intact males. LPS-induced airway hyperresponsiveness was also decreased in castrated males and was increased in females administered exogenous testosterone. Collectively, these data indicate that airway responsiveness in naive mice is influenced by gender, and that male mice have exaggerated airway inflammatory and functional responses to LPS compared with females. These gender differences are mediated, at least in part, by effects of androgens.

This article has been cited by other articles:

				J. J. Reece, M. C. Siracusa, T. L. Southard, C. F. Brayton, J. F. Urban Jr., and A. L. Scott Hookworm-Induced Persistent Changes to the Immunological Environment of the Lung Infect. Immun., August 1, 2008; 76(8): 3511 - 3524. [Abstract] [Full Text] [PDF]

				J. W. Voltz, J. W. Card, M. A. Carey, L. M. DeGraff, C. D. Ferguson, G. P. Flake, J. C. Bonner, K. S. Korach, and D. C. Zeldin Male Sex Hormones Exacerbate Lung Function Impairment after Bleomycin-Induced Pulmonary Fibrosis Am. J. Respir. Cell Mol. Biol., July 1, 2008; 39(1): 45 - 52. [Abstract] [Full Text] [PDF]

				Z. Hammoud, B. Tan, S. Badve, and R. M Bigsby Estrogen promotes tumor progression in a genetically defined mouse model of lung adenocarcinoma Endocr. Relat. Cancer, June 1, 2008; 15(2): 475 - 483. [Abstract] [Full Text] [PDF]

				I. C Villar, A. J Hobbs, and A. Ahluwalia Sex differences in vascular function: implication of endothelium-derived hyperpolarizing factor J. Endocrinol., June 1, 2008; 197(3): 447 - 462. [Abstract] [Full Text] [PDF]

				A. Baldan, A. V. Gomes, P. Ping, and P. A. Edwards Loss of ABCG1 Results in Chronic Pulmonary Inflammation J. Immunol., March 1, 2008; 180(5): 3560 - 3568. [Abstract] [Full Text] [PDF]

				J. C. Cohen, J. Hudak, J. J. Fredberg, Z. Hantos, D. C. Zeldin, J. W. Card, M. A. Carey, J. W. Voltz, B. Suki, K. R. Lutchen, et al. "Lung impedance measurements are/are not more useful than simpler measurements of lung function in animal models of pulmonary disease". J Appl Physiol, November 1, 2007; 103(5): 1905 - 1908. [Full Text] [PDF]

				I. C. Davis, E. R. Lazarowski, F.-P. Chen, J. M. Hickman-Davis, W. M. Sullender, and S. Matalon Post-Infection A77-1726 Blocks Pathophysiologic Sequelae of Respiratory Syncytial Virus Infection Am. J. Respir. Cell Mol. Biol., October 1, 2007; 37(4): 379 - 386. [Abstract] [Full Text] [PDF]

				J. W. Card, J. W. Voltz, M. A. Carey, J. A. Bradbury, L. M. DeGraff, F. B. Lih, J. C. Bonner, D. L. Morgan, G. P. Flake, and D. C. Zeldin Cyclooxygenase-2 Deficiency Exacerbates Bleomycin-Induced Lung Dysfunction but Not Fibrosis Am. J. Respir. Cell Mol. Biol., September 1, 2007; 37(3): 300 - 308. [Abstract] [Full Text] [PDF]

				M. A. Carey, J. W. Card, J. W. Voltz, D. R. Germolec, K. S. Korach, and D. C. Zeldin The impact of sex and sex hormones on lung physiology and disease: lessons from animal studies Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L272 - L278. [Abstract] [Full Text] [PDF]

				J. W. Card, J. W. Voltz, C. D. Ferguson, M. A. Carey, L. M. DeGraff, S. D. Peddada, D. L. Morgan, and D. C. Zeldin Male sex hormones promote vagally mediated reflex airway responsiveness to cholinergic stimulation Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L908 - L914. [Abstract] [Full Text] [PDF]

				J. W. Card, M. A. Carey, J. A. Bradbury, J. P. Graves, F. B. Lih, M. P. Moorman, D. L. Morgan, L. M. DeGraff, Y. Zhao, J. F. Foley, et al. Cyclooxygenase-1 Overexpression Decreases Basal Airway Responsiveness but Not Allergic Inflammation J. Immunol., October 1, 2006; 177(7): 4785 - 4793. [Abstract] [Full Text] [PDF]