The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lahoud, M. H.
Right arrow Articles by Wright, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lahoud, M. H.
Right arrow Articles by Wright, M. D.
The Journal of Immunology, 2006, 177: 372-382.
Copyright © 2006 by The American Association of Immunologists

Signal Regulatory Protein Molecules Are Differentially Expressed by CD8– Dendritic Cells1

Mireille H. Lahoud2,*, Anna I. Proietto*, Kate H. Gartlan{dagger}, Susie Kitsoulis*, Joan Curtis*, James Wettenhall*, Mariam Sofi{dagger}, Carmel Daunt{dagger}, Meredith O’Keeffe*, Irina Caminschi*, Keith Satterley*, Alexandra Rizzitelli*, Petra Schnorrer*, Atsushi Hinohara{ddagger}, Yasunori Yamaguchi{ddagger}, Li Wu*, Gordon Smyth*, Emanuela Handman*, Ken Shortman* and Mark D. Wright{dagger}

* Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; {dagger} MacFarlane Burnet Institute of Medical Research and Public Health, Austin Campus, Heidelberg, Victoria, Australia; and {ddagger} Kirin Brewery, Tokyo, Japan

A normalized subtracted gene expression library was generated from freshly isolated mouse dendritic cells (DC) of all subtypes, then used to construct cDNA microarrays. The gene expression profiles of the three splenic conventional DC (cDC) subsets were compared by microarray hybridization and two genes encoding signal regulatory protein beta (Sirpbeta1 and Sirpbeta4) molecules were identified as differentially expressed in CD8– cDC. Genomic sequence analysis revealed a third Sirpbeta member localized in the same gene cluster. These Sirpbeta genes encode cell surface molecules containing extracellular Ig domains and short intracytoplasmic domains that have a charged amino acid in the transmembrane region which can potentially interact with ITAM-bearing molecules to mediate signaling. Indeed, we demonstrated interactions between Sirpbeta1 and beta2 with the ITAM-bearing signaling molecule Dap12. Real-time PCR analysis showed that all three Sirpbeta genes were expressed by CD8– cDC, but not by CD8+ cDC or plasmacytoid pre-DC. The related Sirp{alpha} gene showed a similar expression profile on cDC subtypes but was also expressed by plasmacytoid pre-DC. The differential expression of Sirp{alpha} and Sirpbeta1 molecules on DC was confirmed by staining with mAbs, including a new mAb recognizing Sirpbeta1. Cross-linking of Sirpbeta1 on DC resulted in a reduction in phagocytosis of Leishmania major parasites, but did not affect phagocytosis of latex beads, perhaps indicating that the regulation of phagocytosis by Sirpbeta1 is a ligand-dependent interaction. Thus, we postulate that the differential expression of these molecules may confer the ability to regulate the phagocytosis of particular ligands to CD8– cDC.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Wirnsberger, F. Mair, and L. Klein
Regulatory T cell differentiation of thymocytes does not require a dedicated antigen-presenting cell but is under T cell-intrinsic developmental control
PNAS, June 23, 2009; 106(25): 10278 - 10283.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. H. Lahoud, A. I. Proietto, F. Ahmet, S. Kitsoulis, L. Eidsmo, L. Wu, P. Sathe, S. Pietersz, H.-W. Chang, I. D. Walker, et al.
The C-Type Lectin Clec12A Present on Mouse and Human Dendritic Cells Can Serve as a Target for Antigen Delivery and Enhancement of Antibody Responses
J. Immunol., June 15, 2009; 182(12): 7587 - 7594.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Nakayama, H. Akiba, K. Takeda, Y. Kojima, M. Hashiguchi, M. Azuma, H. Yagita, and K. Okumura
Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation
Blood, April 16, 2009; 113(16): 3821 - 3830.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Bedoui, S. Prato, J. Mintern, T. Gebhardt, Y. Zhan, A. M. Lew, W. R. Heath, J. A. Villadangos, and E. Segura
Characterization of an Immediate Splenic Precursor of CD8+ Dendritic Cells Capable of Inducing Antiviral T Cell Responses
J. Immunol., April 1, 2009; 182(7): 4200 - 4207.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
B. B. Ganesh, D. M. Cheatem, J. R. Sheng, C. Vasu, and B. S. Prabhakar
GM-CSF-induced CD11c+CD8a--dendritic cells facilitate Foxp3+ and IL-10+ regulatory T cell expansion resulting in suppression of autoimmune thyroiditis
Int. Immunol., March 1, 2009; 21(3): 269 - 282.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
H. K. Lee, M. Zamora, M. M. Linehan, N. Iijima, D. Gonzalez, A. Haberman, and A. Iwasaki
Differential roles of migratory and resident DCs in T cell priming after mucosal or skin HSV-1 infection
J. Exp. Med., February 16, 2009; 206(2): 359 - 370.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
F. Guimont-Desrochers, C. Beauchamp, G. Chabot-Roy, V. Dugas, E. E. Hillhouse, J. Dusseault, G. Langlois, P. Gautier-Ethier, J. Darwiche, M. Sarfati, et al.
Absence of CD47 in vivo influences thymic dendritic cell subset proportions but not negative selection of thymocytes
Int. Immunol., February 1, 2009; 21(2): 167 - 177.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. I. Proietto, S. van Dommelen, P. Zhou, A. Rizzitelli, A. D'Amico, R. J. Steptoe, S. H. Naik, M. H. Lahoud, Y. Liu, P. Zheng, et al.
Dendritic cells in the thymus contribute to T-regulatory cell induction
PNAS, December 16, 2008; 105(50): 19869 - 19874.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M.-L. del Rio, J.-I. Rodriguez-Barbosa, J. Bolter, M. Ballmaier, O. Dittrich-Breiholz, M. Kracht, S. Jung, and R. Forster
CX3CR1+c-kit+ Bone Marrow Cells Give Rise to CD103+ and CD103- Dendritic Cells with Distinct Functional Properties
J. Immunol., November 1, 2008; 181(9): 6178 - 6188.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Kobayashi, H. Ohnishi, H. Okazawa, Y. Murata, Y. Hayashi, H. Kobayashi, T. Kitamura, and T. Matozaki
Expression of Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase Substrate-1 in Pancreatic {beta}-Cells and Its Role in Promotion of Insulin Secretion and Protection against Diabetes
Endocrinology, November 1, 2008; 149(11): 5662 - 5669.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I. Caminschi, A. I. Proietto, F. Ahmet, S. Kitsoulis, J. Shin Teh, J. C. Y. Lo, A. Rizzitelli, L. Wu, D. Vremec, S. L. H. van Dommelen, et al.
The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement
Blood, October 15, 2008; 112(8): 3264 - 3273.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. L. Lin, Y. Zhan, A. I. Proietto, S. Prato, L. Wu, W. R. Heath, J. A. Villadangos, and A. M. Lew
Selective suicide of cross-presenting CD8+ dendritic cells by cytochrome c injection shows functional heterogeneity within this subset
PNAS, February 26, 2008; 105(8): 3029 - 3034.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. K. H. Tan and H. C. O'Neill
Concise Review: Dendritic Cell Development in the Context of the Spleen Microenvironment
Stem Cells, September 1, 2007; 25(9): 2139 - 2145.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.