HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoshioka, Y. Articles by Maeda, S. Search for Related Content PubMed PubMed Citation Articles by Yoshioka, Y. Articles by Maeda, S.

Nitric Oxide Protects Macrophages from Hydrogen Peroxide-Induced Apoptosis by Inducing the Formation of Catalase¹

Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan

We investigated the cytoprotective effect of NO on H₂O₂-induced cell death in mouse macrophage-like cell line RAW264. H₂O₂-treated cells showed apoptotic features, such as activation of caspase-9 and caspase-3, nuclear fragmentation, and DNA fragmentation. These apoptotic features were significantly inhibited by pretreatment for 24 h with NO donors, sodium nitroprusside and 1-hydroxy-2-oxo-3,3-bis-(2-aminoethyl)-1-triazene, at a low nontoxic concentration. The cytoprotective effect of NO was abrogated by the catalase inhibitor 3-amino-1,2,4-triazole but was not affected by a glutathione synthesis inhibitor, L-buthionine-(S,R)-sulfoximine. NO donors increased the level of catalase and its activity in a concentration-dependent manner. Cycloheximide, a protein synthesis inhibitor, inhibited both the NO-induced increase in the catalase level and the cytoprotective effect of NO. These results indicate that NO at a low concentration protects macrophages from H₂O₂-induced apoptosis by inducing the production of catalase.

This article has been cited by other articles:

				K.-S. Choi, E.-K. Song, and C.-Y. Yim Cytokines secreted by IL-2-activated lymphocytes induce endogenous nitric oxide synthesis and apoptosis in macrophages J. Leukoc. Biol., June 1, 2008; 83(6): 1440 - 1450. [Abstract] [Full Text] [PDF]

				R. L. Auten, S. N. Mason, M. H. Whorton, W. R. Lampe, W. M. Foster, R. N. Goldberg, B. Li, J. S. Stamler, and K. M. Auten Inhaled Ethyl Nitrite Prevents Hyperoxia-impaired Postnatal Alveolar Development in Newborn Rats Am. J. Respir. Crit. Care Med., August 1, 2007; 176(3): 291 - 299. [Abstract] [Full Text] [PDF]