The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, K.-J.
Right arrow Articles by Lei, H.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, K.-J.
Right arrow Articles by Lei, H.-Y.
The Journal of Immunology, 2006, 176: 2825-2832.
Copyright © 2006 by The American Association of Immunologists

The Dual-Specific Binding of Dengue Virus and Target Cells for the Antibody-Dependent Enhancement of Dengue Virus Infection1

Kao-Jean Huang*, Yu-Ching Yang{dagger}, Yee-Shin Lin{dagger}, Jyh-Hsiung Huang, Hsiao-Sheng Liu{dagger}, Trai-Ming Yeh{ddagger}, Shun-Hua Chen{dagger}, Ching-Chuan Liu§ and Huan-Yao Lei2,*,{dagger}

* Department of Basic Medical Sciences, {dagger} Department of Microbiology and Immunology, {ddagger} Department of Medical Technology, and § Department of Pediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan; and Center for Disease Control, Department of Health, Taipei, Taiwan, Republic of China

Using flow cytometric assay and monoclonal anti-dengue Ab, we observed that both anti-E and anti-prM Abs could enhance dengue virus infection in a concentration-dependent but serotype-independent manner. Increases were found in both the percentage of dengue-infected cells and the expression of dengue E and NS1 protein per cell. Dengue virion binding and infection were enhanced on FcR-bearing cells via the Fc-Fc{gamma}RII pathway. Furthermore, anti-prM Ab also enhanced dengue virion binding and infection on cells lacking FcR, such as BHK-21 or A549 cells, by the mechanism of peptide (CPFLKQNEPEDIDCW)-specific binding. Anti-prM Ab cross-reacted with BHK-21 or A549 cells and recognized self-Ags such as heat shock protein 60. In summary, a novel mechanism of anti-prM Ab-mediated enhancement on dengue virus infection was found to be mediated by dual specific binding to dengue virion and to target cells, in addition to the traditional enhancement on FcR-bearing cells.




This article has been cited by other articles:


Home page
 J. Virol.Home page
K. Boonnak, B. M. Slike, T. H. Burgess, R. M. Mason, S.-J. Wu, P. Sun, K. Porter, I. F. Rudiman, D. Yuwono, P. Puthavathana, et al.
Role of Dendritic Cells in Antibody-Dependent Enhancement of Dengue Virus Infection
J. Virol., April 15, 2008; 82(8): 3939 - 3951.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. M. van der Schaar, M. J. Rust, B.-L. Waarts, H. van der Ende-Metselaar, R. J. Kuhn, J. Wilschut, X. Zhuang, and J. M. Smit
Characterization of the Early Events in Dengue Virus Cell Entry by Biochemical Assays and Single-Virus Tracking
J. Virol., November 1, 2007; 81(21): 12019 - 12028.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. Oliphant, G. E. Nybakken, M. Engle, Q. Xu, C. A. Nelson, S. Sukupolvi-Petty, A. Marri, B.-E. Lachmi, U. Olshevsky, D. H. Fremont, et al.
Antibody Recognition and Neutralization Determinants on Domains I and II of West Nile Virus Envelope Protein
J. Virol., December 15, 2006; 80(24): 12149 - 12159.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.