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Regulation of IgH Gene Assembly: Role of the Intronic Enhancer and 5'D_Q52 Region in Targeting D_HJ_H Recombination¹

Roshi Afshar^*, Steven Pierce^*, Daniel J. Bolland, Anne Corcoran and Eugene M. Oltz^2,*

^* Department of Microbiology/Immunology, Vanderbilt University Medical School, Nashville, TN 37232; and Laboratory of Chromatin and Gene Expression, The Babraham Institute, Babraham, Cambridge, United Kingdom

The assembly of Ag receptor genes by V(D)J recombination is regulated by transcriptional promoters and enhancers which control chromatin accessibility at Ig and TCR gene segments to the RAG-1/RAG-2 recombinase complex. Paradoxically, germline deletions of the IgH enhancer (Eµ) only modestly reduce D_HJ_H rearrangements when assessed in peripheral B cells. However, deletion of Eµ severely impairs recombination of V_H gene segments, which are located over 100 kb away. We now test two alternative explanations for the minimal effect of Eµ deletions on primary D_HJ_H rearrangement: 1) Accessibility at the D_HJ_H cluster is controlled by a redundant cis-element in the absence of Eµ. One candidate for this element lies 5' to D_Q52 (PD_Q52) and exhibits promoter/enhancer activity in pre-B cells. 2) In contrast to endpoint B cells, D_HJ_H recombination may be significantly impaired in pro-B cells from enhancer-deficient mice. To elucidate the roles of PD_Q52 and Eµ in the regulation of IgH locus accessibility, we generated mice with targeted deletions of these elements. We report that the defined PD_Q52 promoter is dispensable for germline transcription and recombination of the D_HJ_H cluster. In contrast, we demonstrate that Eµ directly regulates accessibility of the D_HJ_H region. These findings reveal a significant role for Eµ in the control mechanisms that activate IgH gene assembly and suggest that impaired V_HD_HJ_H rearrangement in enhancer-deficient cells may be a downstream consequence of the primary block in D_HJ_H recombination.

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