The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Green, S. R.
Right arrow Articles by Quehenberger, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Green, S. R.
Right arrow Articles by Quehenberger, O.
The Journal of Immunology, 2006, 176: 7412-7420.
Copyright © 2006 by The American Association of Immunologists

The CC Chemokine MCP-1 Stimulates Surface Expression of CX3CR1 and Enhances the Adhesion of Monocytes to Fractalkine/CX3CL1 via p38 MAPK1

Simone R. Green2,*, Ki Hoon Han2,3,*, Yiming Chen*, Felicidad Almazan*, Israel F. Charo{dagger}, Yury I. Miller* and Oswald Quehenberger4,*

* Department of Medicine, University of California, San Diego, La Jolla, California 92093; and {dagger} Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141

The membrane-anchored form of CX3CL1 has been proposed as a novel adhesion protein for leukocytes. This functional property of CX3CL1 is mediated through CX3CR1, a chemokine receptor expressed predominantly on circulating white blood cells. Thus far, it is still uncertain at what stage of the trafficking process CX3CR1 becomes importantly involved and how the CX3CR1-dependent adhesion of leukocytes is regulated during inflammation. The objective of this study was to examine the functional effects of chemokine stimulation on CX3CR1-mediated adhesion of human monocytes. Consistent with previous reports, our data indicate that the activity of CX3CR1 on resting monocytes is sufficient to mediate cell adhesion to CX3CL1. However, the basal, nonstimulated adhesion activity is low, and we hypothesized that like the integrins, CX3CR1 may require a preceding activation step to trigger firm leukocyte adhesion. Compatible with this hypothesis, stimulation of monocytes with MCP-1 significantly increased their adhesion to immobilized CX3CL1, under both static and physiological flow conditions. The increase of the adhesion activity was mediated through CCR2-dependent signaling and obligatory activation of the p38 MAPK pathway. Stimulation with MCP-1 also induced a rapid increase of CX3CR1 protein on the cell surface. Inhibition of the p38 MAPK pathway prevented this increase of CX3CR1 surface expression and blunted the effect of MCP-1 on cell adhesion, indicating a causal link between receptor surface density and adhesion activity. Together, our data suggest that a chemokine signal is required for firm CX3CR1-dependent adhesion and demonstrate that CCR2 is an important regulator of CX3CL1-dependent leukocyte adhesion.




This article has been cited by other articles:


Home page
 JCOHome page
M. Rodero, Y. Marie, M. Coudert, E. Blondet, K. Mokhtari, A. Rousseau, W. Raoul, C. Carpentier, F. Sennlaub, P. Deterre, et al.
Polymorphism in the Microglial Cell-Mobilizing CX3CR1 Gene Is Associated With Survival in Patients With Glioblastoma
J. Clin. Oncol., December 20, 2008; 26(36): 5957 - 5964.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Ishida, T. Hayashi, T. Goto, A. Kimura, S. Akimoto, N. Mukaida, and T. Kondo
Essential Involvement of CX3CR1-Mediated Signals in the Bactericidal Host Defense during Septic Peritonitis
J. Immunol., September 15, 2008; 181(6): 4208 - 4218.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Popovic, Y. Laumonnier, L. Burysek, T. Syrovets, and T. Simmet
Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration
J. Leukoc. Biol., July 1, 2008; 84(1): 215 - 223.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Zhao, A. De, Z. Hu, J. Li, S. M. Mulders, M. D. Sollewijn Gelpke, E.-K. Duan, and A. J. W. Hsueh
Gonadotropin Stimulation of Ovarian Fractalkine Expression and Fractalkine Augmentation of Progesterone Biosynthesis by Luteinizing Granulosa Cells
Endocrinology, June 1, 2008; 149(6): 2782 - 2789.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
C. Combadiere, S. Potteaux, M. Rodero, T. Simon, A. Pezard, B. Esposito, R. Merval, A. Proudfoot, A. Tedgui, and Z. Mallat
Combined Inhibition of CCL2, CX3CR1, and CCR5 Abrogates Ly6Chi and Ly6Clo Monocytosis and Almost Abolishes Atherosclerosis in Hypercholesterolemic Mice
Circulation, April 1, 2008; 117(13): 1649 - 1657.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Ishida, J.-L. Gao, and P. M. Murphy
Chemokine Receptor CX3CR1 Mediates Skin Wound Healing by Promoting Macrophage and Fibroblast Accumulation and Function
J. Immunol., January 1, 2008; 180(1): 569 - 579.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
V. Bjerkeli, J. K. Damas, B. Fevang, J. C. Holter, P. Aukrust, and S. S. Froland
Increased expression of fractalkine (CX3CL1) and its receptor, CX3CR1, in Wegener's granulomatosis possible role in vascular inflammation
Rheumatology, September 1, 2007; 46(9): 1422 - 1427.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.