HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sekiguchi, D. R. Articles by Prak, E. T. L. Search for Related Content PubMed PubMed Citation Articles by Sekiguchi, D. R. Articles by Prak, E. T. L. Pubmed/NCBI databases Gene Substance via MeSH

Development and Selection of Edited B Cells in B6.56R Mice¹

Debora R. Sekiguchi^*, Lenka Yunk^*, David Gary, Deepshikha Charan^*, Bhaskar Srivastava^*, David Allman^*, Martin G. Weigert and Eline T. Luning Prak^2,*

^* Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104; and Gwen Knapp Center for Lupus and Autoimmunity Research, University of Chicago, Chicago, IL 60637

Tolerance to dsDNA is broken in mice with a high-affinity anti-DNA H chain transgene, 56R, on the C57BL/6 background (B6.56R). B6.56R produce more anti-dsDNA Abs than BALBc.56R. To investigate how anti-DNA Abs are regulated on the B6 background, phenotypic and genetic studies were performed. B6.56R have reduced numbers of B cells and phenotypically altered B cell subsets, including relative increases in the proportions of IgM-negative bone marrow B cells, cells with a marginal zone phenotype, and cells with a transitional T3 phenotype. The peripheral B cell repertoire in B6.56R is restricted: most B cells express the 56R H chain and use a similar, limited subset of editor L chains. DNA binding is more common in B6.56R because the repertoire is shifted toward L chains that are more permissive for DNA binding. H chain editing is also observed and is increased in spontaneous as compared with LPS hybridomas. A subset of spontaneous hybridomas appears to lack H chain expression.

This article has been cited by other articles:

				Y. Liu, L. Li, and C. Mohan The Role of Rearrangement at the Second Ig Heavy Chain Locus in Maintaining B Cell Tolerance to DNA J. Immunol., June 1, 2008; 180(11): 7721 - 7727. [Abstract] [Full Text] [PDF]

				K. Kiefer, P. B. Nakajima, J. Oshinsky, S. H. Seeholzer, M. Radic, G. C. Bosma, and M. J. Bosma Antigen Receptor Editing in Anti-DNA Transitional B Cells Deficient for Surface IgM J. Immunol., May 1, 2008; 180(9): 6094 - 6106. [Abstract] [Full Text] [PDF]

				E. J. Witsch and E. Bettelheim Allelic and Isotypic Light Chain Inclusion in Peripheral B Cells from Anti-DNA Antibody Transgenic C57BL/6 and BALB/c Mice J. Immunol., March 15, 2008; 180(6): 3708 - 3718. [Abstract] [Full Text] [PDF]

				T. Tarasenko, H. K. Kole, and S. Bolland A Lupus-Suppressor BALB/c Locus Restricts IgG2 Autoantibodies without Altering Intrinsic B Cell-Tolerance Mechanisms J. Immunol., March 15, 2008; 180(6): 3807 - 3814. [Abstract] [Full Text] [PDF]

				S. N. Khan, E. J. Witsch, N. G. Goodman, A. K. Panigrahi, C. Chen, Y. Jiang, A. M. Cline, J. Erikson, M. Weigert, E. T. L. Prak, et al. Editing and escape from editing in anti-DNA B cells PNAS, March 11, 2008; 105(10): 3861 - 3866. [Abstract] [Full Text] [PDF]

				Y. Liu, L. Li, K. R. Kumar, C. Xie, S. Lightfoot, X. J. Zhou, J. F. Kearney, M. Weigert, and C. Mohan Lupus Susceptibility Genes May Breach Tolerance to DNA by Impairing Receptor Editing of Nuclear Antigen-Reactive B Cells J. Immunol., July 15, 2007; 179(2): 1340 - 1352. [Abstract] [Full Text] [PDF]

				C. M. Doyle, J. Han, M. G. Weigert, and E. T. L. Prak Consequences of receptor editing at the {lambda} locus: Multireactivity and light chain secretion PNAS, July 25, 2006; 103(30): 11264 - 11269. [Abstract] [Full Text] [PDF]