HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, C.-C. Articles by Shi, H. N. Search for Related Content PubMed PubMed Citation Articles by Chen, C.-C. Articles by Shi, H. N.

Helminth-Primed Dendritic Cells Alter the Host Response to Enteric Bacterial Infection¹

Chien-Chang Chen^*,, Steve Louie, Beth A. McCormick, W. Allan Walker and Hai Ning Shi^2,

^* Chang Gung Children’s Hospital and Chang Gung University, Taoyuan, Taiwan; and Mucosal Immunology Laboratory, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129

To examine whether intestinal helminth infection may be a risk factor for enteric bacterial infection, a murine model was established using the intestinal helminth Heligomosomoides polygyrus and a murine pathogen Citrobacter rodentium, which causes infectious colitis. Using this model we recently have shown that coinfection with the Th2-inducing H. polygyrus and C. rodentium promotes bacterial-associated disease and colitis. In this study, we expand our previous observations and examine the hypothesis that dendritic cells (DC) stimulated by helminth infection may play an important role in the regulation of the intestinal immune response to concurrent C. rodentium infection as well as in the modulation of the bacterial pathogenesis. We show that H. polygyrus infection induces DC activation and IL-10 expression, and that adoptive transfer of parasite-primed DC significantly impairs host protection to C. rodentium infection, resulting in an enhanced bacterial infection and in the development of a more severe colonic injury. Furthermore, we demonstrate that adoptive transfer of parasite-primed IL-10-deficient DCs fails to result in the development of a significantly enhanced C. rodentium-mediated colitis. Similarly, when the DC IL-10 response was neutralized by anti-IL-10 mAb treatment in mice that received parasite-primed DC, no deleterious effect of the parasite-primed DC on the host intestinal response to C. rodentium was detected. Thus, our results provide evidence to indicate that the H. polygyrus-dependent modulation of the host response to concurrent C. rodentium infection involves IL-10-producing DCs.

This article has been cited by other articles:

				J. Mulvenna, B. Hamilton, S. H. Nagaraj, D. Smyth, A. Loukas, and J. J. Gorman Proteomics Analysis of the Excretory/Secretory Component of the Blood-feeding Stage of the Hookworm, Ancylostoma caninum Mol. Cell. Proteomics, January 1, 2009; 8(1): 109 - 121. [Abstract] [Full Text] [PDF]

				M. E. McBee, P. Z. Zheng, A. B. Rogers, J. G. Fox, and D. B. Schauer Modulation of Acute Diarrheal Illness by Persistent Bacterial Infection Infect. Immun., November 1, 2008; 76(11): 4851 - 4858. [Abstract] [Full Text] [PDF]

				Y. Shimomura, A. Ogawa, M. Kawada, K. Sugimoto, E. Mizoguchi, H.-N. Shi, S. Pillai, A. K. Bhan, and A. Mizoguchi A unique B2 B cell subset in the intestine J. Exp. Med., June 9, 2008; 205(6): 1343 - 1355. [Abstract] [Full Text] [PDF]

				K. N. Couper, D. G. Blount, and E. M. Riley IL-10: The Master Regulator of Immunity to Infection J. Immunol., May 1, 2008; 180(9): 5771 - 5777. [Abstract] [Full Text] [PDF]

				M. P. Hubner, B. Pasche, S. Kalaydjiev, P. T. Soboslay, A. Lengeling, H. Schulz-Key, E. Mitre, and W. H. Hoffmann Microfilariae of the Filarial Nematode Litomosoides sigmodontis Exacerbate the Course of Lipopolysaccharide-Induced Sepsis in Mice Infect. Immun., April 1, 2008; 76(4): 1668 - 1677. [Abstract] [Full Text] [PDF]

				I. A. Khan, R. Hakak, K. Eberle, P. Sayles, L. M. Weiss, and J. F. Urban Jr. Coinfection with Heligmosomoides polygyrus Fails To Establish CD8+ T-Cell Immunity against Toxoplasma gondii Infect. Immun., March 1, 2008; 76(3): 1305 - 1313. [Abstract] [Full Text] [PDF]

				M. Weng, D. Huntley, I-F. Huang, O. Foye-Jackson, L. Wang, A. Sarkissian, Q. Zhou, W. A. Walker, B. J. Cherayil, and H. N. Shi Alternatively Activated Macrophages in Intestinal Helminth Infection: Effects on Concurrent Bacterial Colitis J. Immunol., October 1, 2007; 179(7): 4721 - 4731. [Abstract] [Full Text] [PDF]

				D. Borenshtein, P. R. Nambiar, E. B. Groff, J. G. Fox, and D. B. Schauer Development of Fatal Colitis in FVB Mice Infected with Citrobacter rodentium Infect. Immun., July 1, 2007; 75(7): 3271 - 3281. [Abstract] [Full Text] [PDF]