HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Data Supplement Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Loffredo, J. T. Articles by Watkins, D. I. Search for Related Content PubMed PubMed Citation Articles by Loffredo, J. T. Articles by Watkins, D. I.

The High Frequency Indian Rhesus Macaque MHC Class I Molecule, Mamu-B*01, Does Not Appear to Be Involved in CD8⁺ T Lymphocyte Responses to SIV_mac239¹

John T. Loffredo^*, John Sidney, Shari Piaskowski, Andrew Szymanski, Jessica Furlott^*, Richard Rudersdorf^*, Jason Reed^*, Bjoern Peters, Heather D. Hickman-Miller, Wilfried Bardet, William M. Rehrauer, David H. O’Connor^*, Nancy A. Wilson^*, William H. Hildebrand, Alessandro Sette and David I. Watkins^2,*,

^* Wisconsin National Primate Research Center (WNPRC), University of Wisconsin, Madison, WI 53715; La Jolla Institute for Allergy and Immunology, San Diego, CA 92109; Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53706; and Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104

Although the SIV-infected Indian rhesus macaque (Macaca mulatta) is the animal model most widely used for studying HIV infection, our current understanding of the functional macaque MHC class I molecules is limited. To date, SIV-derived CD8⁺ T lymphocyte epitopes from only three high frequency macaque MHC class I molecules have been extensively characterized. In this study, we defined the peptide-binding properties of the high frequency Indian rhesus macaque class I molecule, Mamu-B*01 (26%). We first identified a preliminary binding motif by eluting and sequencing endogenously bound Mamu-B*01 ligands. We further characterized the peptide-binding characteristics using panels of single amino acid substitution analogs. Using this detailed motif, 507 peptides derived from SIV_mac239 were identified and tested for their Mamu-B*01 binding capacity. Surprisingly, only 11 (2.2%) of these motif-containing peptides bound with IC₅₀ values 500 nM. We assessed the immunogenicity of these peptides using freshly isolated PBMC from ten Mamu-B*01⁺ SIV-infected rhesus macaques in IFN- ELISPOT and IFN-/TNF- intracellular cytokine staining assays. Lymphocytes from these SIV-infected macaques responded to none of these peptides. Furthermore, there was no sequence variation indicative of escape in the regions of the virus that encoded these peptides. Additionally, we could not confirm previous reports of SIV-derived Mamu-B*01-restricted epitopes in the Env and Gag proteins. Our results suggest that the high frequency MHC class I molecule, Mamu-B*01, is not involved in SIV-specific CD8⁺ T lymphocyte responses.

This article has been cited by other articles:

				J. T. Loffredo, J. Maxwell, Y. Qi, C. E. Glidden, G. J. Borchardt, T. Soma, A. T. Bean, D. R. Beal, N. A. Wilson, W. M. Rehrauer, et al. Mamu-B*08-Positive Macaques Control Simian Immunodeficiency Virus Replication J. Virol., August 15, 2007; 81(16): 8827 - 8832. [Abstract] [Full Text] [PDF]

				J. T. Loffredo, B. J. Burwitz, E. G. Rakasz, S. P. Spencer, J. J. Stephany, J. P. Giraldo Vela, S. R. Martin, J. Reed, S. M. Piaskowski, J. Furlott, et al. The Antiviral Efficacy of Simian Immunodeficiency Virus-Specific CD8+ T Cells Is Unrelated to Epitope Specificity and Is Abrogated by Viral Escape J. Virol., March 15, 2007; 81(6): 2624 - 2634. [Abstract] [Full Text] [PDF]

				J. B. Sacha, C. Chung, E. G. Rakasz, S. P. Spencer, A. K. Jonas, A. T. Bean, W. Lee, B. J. Burwitz, J. J. Stephany, J. T. Loffredo, et al. Gag-Specific CD8+ T Lymphocytes Recognize Infected Cells before AIDS-Virus Integration and Viral Protein Expression J. Immunol., March 1, 2007; 178(5): 2746 - 2754. [Abstract] [Full Text] [PDF]