HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mulder, A. Articles by Claas, F. H. J. Search for Related Content PubMed PubMed Citation Articles by Mulder, A. Articles by Claas, F. H. J.

Impact of Peptides on the Recognition of HLA Class I Molecules by Human HLA Antibodies

Arend Mulder^1,*, Chantal Eijsink^*, Michel G. D. Kester, Marry E. I. Franke^*, Marrie J. Kardol^*, Mirjam H. M. Heemskerk, Cees van Kooten, Frank A. Verreck^2,*, Jan Wouter Drijfhout^*, Frits Koning^*, Ilias I. N. Doxiadis^* and Frans H. J. Claas^*

^* Department of Immunohematology and Bloodtransfusion, Department of Experimental Hematology, and Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands

MHC class I molecules expressed on cell surfaces are composed of H chain, ₂-microglobulin and any of a vast array of peptides. The role of peptide in the recognition of HLA class I by serum HLA Abs is unknown. In this study, the solid-phase assay of a series (n = 11) of HLA-A2-reactive, pregnancy-induced, human mAbs on a panel (n = 12) of recombinant monomeric HLA-A2 molecules, each containing a single peptide, revealed peptide selectivity of the mAbs. The flow cytometry membrane staining intensities on the HLA-A2-transduced cell line K562, caused by these mAbs, correlated with the number of monomer species detected by the mAbs. Flow cytometry staining on HLA-A2-bearing cell lines of a variety of lineages was indicative of tissue selectivity of these HLA-A2 mAbs. This tissue selectivity suggests that the deleterious effect on allografts is confined to alloantibodies recognizing only HLA class I loaded with peptides that are derived from tissue-specific and household proteins. Since Abs that are only reactive with HLA loaded with irrelevant peptides are expected to be harmless toward allografts, the practice of HLA Ab determination on lymphocyte-derived HLA deserves reconsideration.

This article has been cited by other articles:

				A. Martayan, L. Sibilio, E. Tremante, E. L. Monaco, A. Mulder, D. Fruci, A. Cova, L. Rivoltini, and P. Giacomini Class I HLA Folding and Antigen Presentation in {beta}2-Microglobulin-Defective Daudi Cells J. Immunol., March 15, 2009; 182(6): 3609 - 3617. [Abstract] [Full Text] [PDF]

				J. Racape, F. Connan, J. Hoebeke, J. Choppin, and J.-G. Guillet Influence of dominant HIV-1 epitopes on HLA-A3/peptide complex formation PNAS, November 28, 2006; 103(48): 18208 - 18213. [Abstract] [Full Text] [PDF]