The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schroeder, J. T.
Right arrow Articles by Liu, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schroeder, J. T.
Right arrow Articles by Liu, M. C.
The Journal of Immunology, 2005, 175: 5724-5731.
Copyright © 2005 by The American Association of Immunologists

TLR9- and Fc{epsilon}RI-Mediated Responses Oppose One Another in Plasmacytoid Dendritic Cells by Down-Regulating Receptor Expression1

John T. Schroeder2,*, Anja P. Bieneman*, HuiQing Xiao{dagger}, Kristin L. Chichester*, Kavitha Vasagar*, Sarbjit Saini* and Mark C. Liu*,{dagger}

Department of Medicine, Divisions of * Clinical Immunology and {dagger} Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University, Baltimore, MD 21224

Plasmacytoid dendritic cells (pDC) express not only TLR9 molecules through which ligation with CpG DNA favors Th1 responses but also possess IgE receptors (Fc{epsilon}RI) implicated in allergen presentation and induction of Th2 responses. This dichotomy prompted an investigation to determine whether TLR9- and IgE receptor-mediated responses oppose one another in pDC by affecting receptor expression and associated functional responses. Results showed that IgE cross-linking reduced TLR9 in pDC and inhibited the capacity of these cells to secrete IFN-{alpha} when stimulated with the CpG oligodeoxynucleotide (ODN)-2216. In contrast, an ~15-fold reduction in Fc{epsilon}RI{alpha} mRNA and a loss in surface protein were seen in pDC first exposed to TLR9 ligation with ODN-2216. Results indicated that type I IFNs partly mediated this effect, as rIFN-{alpha} also caused a significant ~4-fold reduction in Fc{epsilon}RI{alpha} mRNA. Finally, this reduction in Fc{epsilon}RI{alpha} mediated by ODN-2216 correlated with a selective suppression of allergen-induced CD4+ T cell proliferation, but not of responses resulting from tetanus toxoid. Overall, these results imply mechanisms by which specific innate and IgE-dependent immune responses counterregulate one another at the dendritic cell level and may have significant impact on whether an ensuing response is either of Th1 or Th2 in nature.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. T. Schroeder, K. L. Chichester, and A. P. Bieneman
Human Basophils Secrete IL-3: Evidence of Autocrine Priming for Phenotypic and Functional Responses in Allergic Disease
J. Immunol., February 15, 2009; 182(4): 2432 - 2438.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Benitez-Ribas, P. Tacken, C. J. A. Punt, I. J. M. de Vries, and C. G. Figdor
Activation of Human Plasmacytoid Dendritic Cells by TLR9 Impairs Fc{gamma}RII-Mediated Uptake of Immune Complexes and Presentation by MHC Class II
J. Immunol., October 15, 2008; 181(8): 5219 - 5224.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. J. Kim, M. Caton, C. Wang, M. Khalil, Z.-J. Zhou, J. Hardin, and B. Diamond
Increased IL-12 inhibits B cells' differentiation to germinal center cells and promotes differentiation to short-lived plasmablasts
J. Exp. Med., September 29, 2008; 205(10): 2437 - 2448.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
X. Ju, M. Zenke, D. N. J. Hart, and G. J. Clark
CD300a/c regulate type I interferon and TNF-{alpha} secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands
Blood, August 15, 2008; 112(4): 1184 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. H. Grayson, D. Cheung, M. M. Rohlfing, R. Kitchens, D. E. Spiegel, J. Tucker, J. T. Battaile, Y. Alevy, L. Yan, E. Agapov, et al.
Induction of high-affinity IgE receptor on lung dendritic cells during viral infection leads to mucous cell metaplasia
J. Exp. Med., October 29, 2007; 204(11): 2759 - 2769.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. K. R. Karaolis, T. K. Means, D. Yang, M. Takahashi, T. Yoshimura, E. Muraille, D. Philpott, J. T. Schroeder, M. Hyodo, Y. Hayakawa, et al.
Bacterial c-di-GMP Is an Immunostimulatory Molecule
J. Immunol., February 15, 2007; 178(4): 2171 - 2181.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
K. Bratke, M. Lommatzsch, P. Julius, M. Kuepper, H.-D. Kleine, W. Luttmann, and J Christian Virchow
Dendritic cell subsets in human bronchoalveolar lavage fluid after segmental allergen challenge
Thorax, February 1, 2007; 62(2): 168 - 175.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
W. Cao, D. B. Rosen, T. Ito, L. Bover, M. Bao, G. Watanabe, Z. Yao, L. Zhang, L. L. Lanier, and Y.-J. Liu
Plasmacytoid dendritic cell-specific receptor ILT7-Fc{varepsilon}RI{gamma} inhibits Toll-like receptor-induced interferon production
J. Exp. Med., June 12, 2006; 203(6): 1399 - 1405.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.