| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(ER) Deficiency in Macrophages Results in Increased Stimulation of CD4+ T Cells while 17
-Estradiol Acts through ER to Increase IL-4 and GATA-3 Expression in CD4+ T Cells Independent of Antigen Presentation1
,,
,,
* Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65211;
Department of Pediatrics, University of Texas Medical Branch, Sealy Center for Vaccine Development, Galveston, TX 77555;
Department of Biochemistry, University of Missouri, Columbia, MO 65211; and
University of Missouri Center for Phytonutrient and Phytochemical Research, Columbia, MO 65211
The effects of 17
-estradiol (E2) on immune function have been extensively reported. The effects are dependent on concentration and duration of exposure and potential differences in signaling between the known E2 receptors, estrogen receptors (ER) 
and ER. Through the use of ER-deficient mice, we and others have begun to demonstrate the role of the two known receptors in modulating immune functional activities. Previous studies have shown that cells of the innate immune system have altered function (bactericidal capacity) and patterns of cytokine expression (increased proinflammatory cytokine expression) through amelioration of ER signaling. In this study, we extend these studies to analysis of T cell differentiation and proliferation in APC-dependent and APC-independent in vitro assay systems. Our results demonstrate that ER deficiency in splenic macrophages, but not CD11c+ splenic dendritic cells pulsed with OVA significantly enhances proliferative responses and IFN-
production by transgenic OVA peptide-specific (OT-II) CD4+ T cells when compared with Ag-pulsed APC from wild-type littermates. The addition of E2 in this culture system did not significantly affect the production of IFN-. In addition, when purified CD4+ T cells from ER-deficient and wild-type littermates were stimulated with anti-CD3/CD28 Ab in the absence of E2, there were no significant differences in IFN- or IL-4 production. However, the addition of E2 significantly increased IL-4 secretion, as well as increased GATA-3 mRNA levels from ER-replete CD4+ T cells, while this effect was abrogated in ER-deficient CD4+ T cells.
This article has been cited by other articles:
|
|
 |
|
  M. M. Escribese, T. Kraus, E. Rhee, A. Fernandez-Sesma, C. B. Lopez, and T. M. Moran Estrogen inhibits dendritic cell maturation to RNA viruses Blood, December 1, 2008; 112(12): 4574 - 4584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hong, T. Wang, C. Huang, W. Cheng, and B. Lin Soy isoflavones supplementation alleviates disease severity in autoimmune-prone MRL-lpr/lpr mice Lupus, September 1, 2008; 17(9): 814 - 821. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Inman, K. Kawana, D. Schust, R. Lininger, and S. Young Cyclic Regulation of T-Bet and GATA-3 in Human Endometrium Reproductive Sciences, January 1, 2008; 15(1): 83 - 90. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Suzuki, T. Shimizu, H.-P. Yu, Y.-C. Hsieh, M. A. Choudhry, K. I. Bland, and I. H. Chaudry Estrogen receptor-{alpha} predominantly mediates the salutary effects of 17beta-estradiol on splenic macrophages following trauma-hemorrhage Am J Physiol Cell Physiol, September 1, 2007; 293(3): C978 - C984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. L. de Oliveira, H. V. Domingos, G. Cavriani, A. S. Damazo, A. L. dos Santos Franco, S. M. Oliani, R. M. Oliveira-Filho, B. B. Vargaftig, and W. T. de Lima Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1120 - C1128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Straub The Complex Role of Estrogens in Inflammation Endocr. Rev., August 1, 2007; 28(5): 521 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Matson, L. Zhu, E. G. Lingenheld, C. M. Schramm, R. B. Clark, D. M. Selander, R. S. Thrall, E. Breen, and L. Puddington Maternal Transmission of Resistance to Development of Allergic Airway Disease J. Immunol., July 15, 2007; 179(2): 1282 - 1291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suzuki, T. Shimizu, H.-P. Yu, Y.-C. Hsieh, M. A. Choudhry, and I. H. Chaudry Salutary effects of 17beta-estradiol on T-cell signaling and cytokine production after trauma-hemorrhage are mediated primarily via estrogen receptor-{alpha} Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2103 - C2111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Polanczyk, C. Hopke, A. A. Vandenbark, and H. Offner Treg suppressive activity involves estrogen-dependent expression of programmed death-1 (PD-1) Int. Immunol., March 1, 2007; 19(3): 337 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Suzuki, T. Shimizu, H.-P. Yu, Y.-C. Hsieh, M. A. Choudhry, M. G. Schwacha, and I. H. Chaudry Tissue compartment-specific role of estrogen receptor subtypes in immune cell cytokine production following trauma-hemorrhage J Appl Physiol, January 1, 2007; 102(1): 163 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Marriott, K. R. McGann-Gramling, B. Hauss-Wegrzyniak, L. C. Sheldahl, R. A. Shapiro, D. M. Dorsa, and G. L. Wenk Brain Infusion of Lipopolysaccharide Increases Uterine Growth as a Function of Estrogen Replacement Regimen: Suppression of Uterine Estrogen Receptor-{alpha} by Constant, But Not Pulsed, Estrogen Replacement Endocrinology, January 1, 2007; 148(1): 232 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Hildebrand, W. J. Hubbard, M. A. Choudhry, B. M. Thobe, H.-C. Pape, and I. H. Chaudry Are the protective effects of 17{beta}-estradiol on splenic macrophages and splenocytes after trauma-hemorrhage mediated via estrogen-receptor (ER)-{alpha} or ER-{beta}? J. Leukoc. Biol., June 1, 2006; 79(6): 1173 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wang, P. Crisostomo, G. M. Wairiuko, and D. R. Meldrum Estrogen receptor-{alpha} mediates acute myocardial protection in females Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2204 - H2209. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
