The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Loscher, C. E.
Right arrow Articles by Roche, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loscher, C. E.
Right arrow Articles by Roche, H. M.
Right arrowPubmed/NCBI databases
*Substance via MeSH
The Journal of Immunology, 2005, 175: 4990-4998.
Copyright © 2005 by The American Association of Immunologists

Conjugated Linoleic Acid Suppresses NF-{kappa}B Activation and IL-12 Production in Dendritic Cells through ERK-Mediated IL-10 Induction1

Christine E. Loscher*, Eve Draper*, Olive Leavy{dagger}, Dermot Kelleher*, Kingston H. G. Mills2,{dagger} and Helen M. Roche2,3,*

* Department of Clinical Medicine, Institute of Molecular Medicine and {dagger} Immune Regulation Research Group, Department of Biochemistry, Trinity College, Dublin, Ireland

Polyunsaturated fatty acids (PUFA) have been shown to modulate immune responses and have therapeutic effects in inflammatory disorders. However, the influence of PUFA on dendritic cells (DC), key cells of the innate immune system in shaping adaptive immune responses, has not yet been defined. In this study, we examine the effects of the cis-9, trans-11 isomer of conjugated linoleic acid (c9, t11-CLA), a dietary PUFA found in meat and dairy products, on murine DC activation. Treatment of DC with c9, t11-CLA suppressed LPS-induced IL-12, enhanced IL-10R expression, and enhanced IL-10 production at the transcriptional and protein level. The suppression of IL-12 by c9, t11-CLA was found to be IL-10 dependent. We investigated the involvement of the MAPK, ERK, and the transcription factor, NF-{kappa}B, in this IL-10-mediated effect. c9, t11-CLA enhanced ERK activation after LPS stimulation, and inhibition of ERK resulted in abrogation of IL-10 and recovery of IL-12 production. c9, t11-CLA decreased NF-{kappa}B:DNA binding after LPS stimulation, which was concomitant with delayed translocation of NF-{kappa}Bp65 into the nucleus and an increase in I{kappa}B{alpha}. These effects were reversed by addition of a neutralizing anti-IL-10 Ab. Our findings demonstrate that c9, t11-CLA suppresses IL-12 production by LPS-stimulated DC by ERK mediated IL-10-induction. Furthermore, these IL-10-mediated effects are dependent on inhibition of NF-{kappa}B activation. This is the first study to demonstrate that c9, t11-CLA can enhance transcription and production of the anti-inflammatory cytokine IL-10, while inhibiting the Th1-promoting cytokine IL-12, and may explain certain of its immunosuppressive properties.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
F. B. Hickey, C. F. Brereton, and K. H. G. Mills
Adenylate cycalse toxin of Bordetella pertussis inhibits TLR-induced IRF-1 and IRF-8 activation and IL-12 production and enhances IL-10 through MAPK activation in dendritic cells
J. Leukoc. Biol., July 1, 2008; 84(1): 234 - 243.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. V. Poncini, C. D. A. Soto, E. Batalla, M. E. Solana, and S. M. Gonzalez Cappa
Trypanosoma cruzi Induces Regulatory Dendritic Cells In Vitro
Infect. Immun., June 1, 2008; 76(6): 2633 - 2641.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
A. Kennedy, S. Chung, K. LaPoint, O. Fabiyi, and M. K. McIntosh
Trans-10, Cis-12 Conjugated Linoleic Acid Antagonizes Ligand-Dependent PPAR{gamma} Activity in Primary Cultures of Human Adipocytes
J. Nutr., March 1, 2008; 138(3): 455 - 461.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
F. Moloney, S. Toomey, E. Noone, A. Nugent, B. Allan, C. E. Loscher, and H. M. Roche
Antidiabetic Effects of cis-9, trans-11-Conjugated Linoleic Acid May Be Mediated via Anti-Inflammatory Effects in White Adipose Tissue
Diabetes, March 1, 2007; 56(3): 574 - 582.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
D.-M. Hwang, J. K. Kundu, J.-W. Shin, J.-C. Lee, H. J. Lee, and Y.-J. Surh
cis-9,trans-11-Conjugated linoleic acid down-regulates phorbol ester-induced NF-{kappa}B activation and subsequent COX-2 expression in hairless mouse skin by targeting I{kappa}B kinase and PI3K-Akt
Carcinogenesis, February 1, 2007; 28(2): 363 - 371.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
P. Bergamo, D. Luongo, F. Maurano, G. Mazzarella, R. Stefanile, and M. Rossi
Conjugated linoleic acid enhances glutathione synthesis and attenuates pathological signs in MRL/MpJ-Faslpr mice
J. Lipid Res., November 1, 2006; 47(11): 2382 - 2391.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Ito, Y.-H. Wang, O. Duramad, S. Hanabuchi, O. A. Perng, M. Gilliet, F. X.-F. Qin, and Y.-J. Liu
OX40 ligand shuts down IL-10-producing regulatory T cells
PNAS, August 29, 2006; 103(35): 13138 - 13143.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.