| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Immunotechnology, Lund University, Lund, Sweden
Human dendritic cells (DCs) are a distinct but heterogeneous lineage of APCs operating as the link between innate and adaptive immune responses, with the function to either maintain tolerance or trigger immunity. The DC lineage consists of several subpopulations with unique phenotypes; however, their functional characteristics and transcriptional similarities remain largely unknown. To further characterize the phenotypes and transcriptomes of the subsets, we purified myeloid CD16+, blood DC Ag 1+ (BDCA1+), and BDCA3+ DC populations, as well as plasmacytoid CD123+ DCs, from tonsillar tissue and peripheral blood. Transcriptional profiling and hierarchical clustering visualized that BDCA1+ DCs clustered with BDCA3+ DCs, whereas CD16+ DCs and CD123+ DCs clustered as distinct populations in blood. Differential expression levels of chemokines, ILs, and pattern recognition receptors were demonstrated, which emphasize innate DC subset specialization. Even though highly BDCA1+ and BDCA3+ DC-specific gene expression was identified in blood, the BDCA1+ DCs and BDCA3+ DCs from tonsils displayed similar transcriptional activity, most likely due to the pathogenic or inflammatory maturational signals present in tonsillar tissues. Of note, plasmacytoid DCs displayed less plasticity in their transcriptional activity compared with myeloid DCs. The data demonstrated a functionally distinct association of each of the seven subsets based on their signatures, involving regulatory genes in adaptive and innate immunity.
This article has been cited by other articles:
|
|
 |
|
  V. Veckman and I. Julkunen Streptococcus pyogenes activates human plasmacytoid and myeloid dendritic cells J. Leukoc. Biol., February 1, 2008; 83(2): 296 - 304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Piccioli, S. Tavarini, E. Borgogni, V. Steri, S. Nuti, C. Sammicheli, M. Bardelli, D. Montagna, F. Locatelli, and A. Wack Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets Blood, June 15, 2007; 109(12): 5371 - 5379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Urban, D. Cordery, M. J. Shafi, P. C. Bull, C. I. Newbold, T. N. Williams, and K. Marsh The Frequency of BDCA3-Positive Dendritic Cells Is Increased in the Peripheral Circulation of Kenyan Children with Severe Malaria Infect. Immun., December 1, 2006; 74(12): 6700 - 6706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. K. Demedts, K. R. Bracke, T. Maes, G. F. Joos, and G. G. Brusselle Different Roles for Human Lung Dendritic Cell Subsets in Pulmonary Immune Defense Mechanisms Am. J. Respir. Cell Mol. Biol., September 1, 2006; 35(3): 387 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-X. Hubert, C. Voisine, C. Louvet, J.-M. Heslan, A. Ouabed, M. Heslan, and R. Josien Differential Pattern Recognition Receptor Expression but Stereotyped Responsiveness in Rat Spleen Dendritic Cell Subsets J. Immunol., July 15, 2006; 177(2): 1007 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Abbasian, D. Mahmud, N. Mahmud, S. Chunduri, H. Araki, P. Reddy, R. Hoffman, M. Arpinati, J. L. M. Ferrara, and D. Rondelli Allogeneic T cells induce rapid CD34+ cell differentiation into CD11c+CD86+ cells with direct and indirect antigen-presenting function Blood, July 1, 2006; 108(1): 203 - 208. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
