| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* National Institute of Immunology, New Delhi, India;
International Centre for Genetic Engineering and Biotechnology, New Delhi, India; and
National Centre for Biological Sciences, Bangalore, India
The Nef protein of HIV-1 is essential for its pathogenicity and is known to down-regulate MHC expression on infected cell surfaces. We now show that Nef also redistributes the costimulatory molecules CD80 and CD86 away from the cell surface in the human monocytic U937 cell line as well as in mouse macrophages and dendritic cells. Furthermore, HIV-1-infected U937 cells and human blood-derived macrophages show a similar loss of cell surface CD80 and CD86. Nef colocalizes with MHC class I (MHCI), CD80, and CD86 in intracellular compartments, and binds to both mouse and human CD80 and CD86. Some Nef mutants defective in MHCI down-modulation, including one from a clinical isolate, remain capable of down-modulating CD80 and CD86. Nef-mediated loss of surface CD80/CD86 is functionally significant, because it leads to compromised activation of naive T cells. This novel immunomodulatory role of Nef may be of potential importance in explaining the correlations of macrophage-tropism and Nef with HIV-1 pathogenicity and immune evasion.
This article has been cited by other articles:
|
|
 |
|
  A. Chaudhry, D. A. Verghese, S. R. Das, S. Jameel, A. George, V. Bal, S. Mayor, and S. Rath HIV-1 Nef Promotes Endocytosis of Cell Surface MHC Class II Molecules via a Constitutive Pathway J. Immunol., August 15, 2009; 183(4): 2415 - 2424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Salek-Ardakani, R. Arens, R. Flynn, A. Sette, S. P. Schoenberger, and M. Croft Preferential Use of B7.2 and Not B7.1 in Priming of Vaccinia Virus-Specific CD8 T Cells J. Immunol., March 1, 2009; 182(5): 2909 - 2918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kumari, V. Borroni, A. Chaudhry, B. Chanda, R. Massol, S. Mayor, and F. J. Barrantes Nicotinic acetylcholine receptor is internalized via a Rac-dependent, dynamin-independent endocytic pathway J. Cell Biol., October 22, 2008; 181(7): 1179 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hussain, C. Wesley, M. Khalid, A. Chaudhry, and S. Jameel Human Immunodeficiency Virus Type 1 Vpu Protein Interacts with CD74 and Modulates Major Histocompatibility Complex Class II Presentation J. Virol., January 15, 2008; 82(2): 893 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Basu, S. Bhaumik, A. K. Haldar, K. Naskar, T. De, S. K. Dana, P. Walden, and S. Roy Hybrid Cell Vaccination Resolves Leishmania donovani Infection by Eliciting a Strong CD8+ Cytotoxic T-Lymphocyte Response with Concomitant Suppression of Interleukin-10 (IL-10) but Not IL-4 or IL-13 Infect. Immun., December 1, 2007; 75(12): 5956 - 5966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Borowski, A. C. Boesteanu, Y. M. Mueller, C. Carafides, D. J. Topham, J. D. Altman, S. R. Jennings, and P. D. Katsikis Memory CD8+ T Cells Require CD28 Costimulation J. Immunol., November 15, 2007; 179(10): 6494 - 6503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Venzke, N. Michel, I. Allespach, O. T. Fackler, and O. T. Keppler Expression of Nef Downregulates CXCR4, the Major Coreceptor of Human Immunodeficiency Virus, from the Surfaces of Target Cells and Thereby Enhances Resistance to Superinfection J. Virol., November 15, 2006; 80(22): 11141 - 11152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Quaranta, B. Mattioli, L. Giordani, and M. Viora The immunoregulatory effects of HIV-1 Nef on dendritic cells and the pathogenesis of AIDS FASEB J, November 1, 2006; 20(13): 2198 - 2208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Brown, H. Zhang, P. Lopez, C. A. Pardo, and S. Gartner In vitro modeling of the HIV-macrophage reservoir J. Leukoc. Biol., November 1, 2006; 80(5): 1127 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Michel, K. Ganter, S. Venzke, J. Bitzegeio, O. T. Fackler, and O. T. Keppler The Nef Protein of Human Immunodeficiency Virus Is a Broad-Spectrum Modulator of Chemokine Receptor Cell Surface Levels That Acts Independently of Classical Motifs for Receptor Endocytosis and G{alpha}i Signaling Mol. Biol. Cell, August 1, 2006; 17(8): 3578 - 3590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Roeth and K. L. Collins Human Immunodeficiency Virus Type 1 Nef: Adapting to Intracellular Trafficking Pathways Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 548 - 563. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
