HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Abe, N. Articles by Satake, M. Search for Related Content PubMed PubMed Citation Articles by Abe, N. Articles by Satake, M.

Reduction of Runx1 Transcription Factor Activity Up-Regulates Fas and Bim Expression and Enhances the Apoptotic Sensitivity of Double Positive Thymocytes¹

Natsumi Abe^*,, Kazuyoshi Kohu^*, Hidetaka Ohmori^*, Keitaro Hayashi^*, Toshio Watanabe^*, Katsuto Hozumi, Takehito Sato, Sonoko Habu and Masanobu Satake^2,*

^* Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan; and Department of Immunology, Tokai University School of Medicine, Isehara, Japan

The death or survival of double positive (DP) thymocytes is determined by the strength of their TCR signaling. Of the three Runx family proteins, the DP cells only express the Runx1 transcription factor. We introduced and expressed in murine thymocytes the Runt domain of Runx1, which antagonizes the activity of endogenous Runx1. The Runt transgenic DP thymocytes expressed higher levels of the proapoptotic molecules Fas and Bim compared with the wild-type cells. Furthermore, the Runt transgenic cells were more susceptible to apoptosis induced by the artificial cross-linking of the TCR by the anti-CD3 Ab. This susceptibility was partially abrogated by the lpr/lpr background. In addition, Runx1:HY-TCR-double transgenic DP thymocytes were resistant to the apoptosis induced by the endogenously presented HY Ag. We propose that Runx1 functions to suppress the apoptotic sensitivity of DP thymocytes in the context of TCR signaling.

This article has been cited by other articles:

				J. H. Carter, J. M. Lefebvre, D. L. Wiest, and W. G. Tourtellotte Redundant Role for Early Growth Response Transcriptional Regulators in Thymocyte Differentiation and Survival J. Immunol., June 1, 2007; 178(11): 6796 - 6805. [Abstract] [Full Text] [PDF]