| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases, Rockefeller University, New York, NY 10021;
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029; and
Department of Dermatology and Venereology, University Hospital of Geneva, Geneva, Switzerland
The C-type lectin dendritic cell-specific ICAM 3-grabbing nonintegrin (DC-SIGN)/CD209 efficiently binds several pathogens, including HIV-1. DC-SIGN is expressed on monocyte-derived DCs in culture, and importantly, it is able to sequester HIV-1 within cells and facilitate transmission of virus to CD4+ T cells. To investigate DC-SIGN function, we have generated new mAbs. We report in this study that these and prior anti-DC-SIGN mAbs primarily label macrophages in the medullary sinuses of noninflamed human lymph node. In contrast, expression is not detected on most DCs in the T cell area, except for occasional cells. We also noted that IL-4 alone can induce expression of DC-SIGN in CD14+ monocytes and circulating blood DCs. However, blockade of DC-SIGN with Abs and DC-SIGN small interfering RNA did not result in a major reduction in the capacity of these DCs to transfer HIV to T cells, confirming significant DC-SIGN-independent mechanisms. The blocking approaches did reduce HIV-1 transmission by DC-SIGN-transfected cells by >90%. DC-SIGN blockade also did not reduce the ability of DCs to stimulate T cell proliferation in the MLR. These results indicate that DC-SIGN has the potential to contribute to macrophage function in normal human lymph node, and that DCs do not require DC-SIGN to transmit HIV or to initiate T cell responses.
This article has been cited by other articles:
|
|
 |
|
  P. Zhang, M. Skurnik, S.-S. Zhang, O. Schwartz, R. Kalyanasundaram, S. Bulgheresi, J. J. He, J. D. Klena, B. J. Hinnebusch, and T. Chen Human Dendritic Cell-Specific Intercellular Adhesion Molecule-Grabbing Nonintegrin (CD209) Is a Receptor for Yersinia pestis That Promotes Phagocytosis by Dendritic Cells Infect. Immun., May 1, 2008; 76(5): 2070 - 2079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Higgins, J. Sanchez-Campillo, A. G. Rosas-Taraco, J. R. Higgins, E. J. Lee, I. M. Orme, and M. Gonzalez-Juarrero Relative Levels of M-CSF and GM-CSF Influence the Specific Generation of Macrophage Populations during Infection with Mycobacterium tuberculosis J. Immunol., April 1, 2008; 180(7): 4892 - 4900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Neumann, N. L. Thompson, and K. Jacobson Distribution and lateral mobility of DC-SIGN on immature dendritic cells-implications for pathogen uptake J. Cell Sci., March 1, 2008; 121(5): 634 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kawamura, Y. Koyanagi, Y. Nakamura, Y. Ogawa, A. Yamashita, T. Iwamoto, M. Ito, A. Blauvelt, and S. Shimada Significant Virus Replication in Langerhans Cells following Application of HIV to Abraded Skin: Relevance to Occupational Transmission of HIV J. Immunol., March 1, 2008; 180(5): 3297 - 3304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Trumpfheller, M. Caskey, G. Nchinda, M. P. Longhi, O. Mizenina, Y. Huang, S. J. Schlesinger, M. Colonna, and R. M. Steinman The microbial mimic poly IC induces durable and protective CD4+ T cell immunity together with a dendritic cell targeted vaccine PNAS, February 19, 2008; 105(7): 2574 - 2579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Duvall, K. Lore, H. Blaak, D. A. Ambrozak, W. C. Adams, K. Santos, C. Geldmacher, J. R. Mascola, A. J. McMichael, A. Jaye, et al. Dendritic Cells Are Less Susceptible to Human Immunodeficiency Virus Type 2 (HIV-2) Infection than to HIV-1 Infection J. Virol., December 15, 2007; 81(24): 13486 - 13498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Lee, P. A. Sieling, M. T. Ochoa, S. R. Krutzik, B. Guo, M. Hernandez, T. H. Rea, G. Cheng, M. Colonna, and R. L. Modlin LILRA2 Activation Inhibits Dendritic Cell Differentiation and Antigen Presentation to T Cells J. Immunol., December 15, 2007; 179(12): 8128 - 8136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. de Witte, M. Bobardt, U. Chatterji, G. Degeest, G. David, T. B. H. Geijtenbeek, and P. Gallay Syndecan-3 is a dendritic cell-specific attachment receptor for HIV-1 PNAS, December 4, 2007; 104(49): 19464 - 19469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Mayer, U. M. Irschick, P. Moser, M. Wurm, H. P. Huemer, N. Romani, and E. U. Irschick Characterization of Antigen-Presenting Cells in Fresh and Cultured Human Corneas Using Novel Dendritic Cell Markers Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4459 - 4467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Zhang, P. Colmenero, U. Purath, C. Teixeira de Matos, W. Hueber, L. Klareskog, I. H. Tarner, E. G. Engleman, and K. Soderstrom Natural killer cells trigger differentiation of monocytes into dendritic cells Blood, October 1, 2007; 110(7): 2484 - 2493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Wang, A. M. Janas, W. J. Olson, and L. Wu Functionally Distinct Transmission of Human Immunodeficiency Virus Type 1 Mediated by Immature and Mature Dendritic Cells J. Virol., September 1, 2007; 81(17): 8933 - 8943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W.-P. Hong, S. Nguyen, S. Young, S. V. Su, and B. Lee Identification of the Optimal DC-SIGN Binding Site on Human Immunodeficiency Virus Type 1 gp120 J. Virol., August 1, 2007; 81(15): 8325 - 8336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Izquierdo-Useros, J. Blanco, I. Erkizia, M. T. Fernandez-Figueras, F. E. Borras, M. Naranjo-Gomez, M. Bofill, L. Ruiz, B. Clotet, and J. Martinez-Picado Maturation of Blood-Derived Dendritic Cells Enhances Human Immunodeficiency Virus Type 1 Capture and Transmission J. Virol., July 15, 2007; 81(14): 7559 - 7570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dominguez-Soto, L. Aragoneses-Fenoll, E. Martin-Gayo, L. Martinez-Prats, M. Colmenares, M. Naranjo-Gomez, F. E. Borras, P. Munoz, M. Zubiaur, M. L. Toribio, et al. The DC-SIGN-related lectin LSECtin mediates antigen capture and pathogen binding by human myeloid cells Blood, June 15, 2007; 109(12): 5337 - 5345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Soares, H. Waechter, N. Glaichenhaus, E. Mougneau, H. Yagita, O. Mizenina, D. Dudziak, M. C. Nussenzweig, and R. M. Steinman A subset of dendritic cells induces CD4+ T cells to produce IFN-{gamma} by an IL-12-independent but CD70-dependent mechanism in vivo J. Exp. Med., May 14, 2007; 204(5): 1095 - 1106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Wang, A. M. Janas, W. J. Olson, V. N. KewalRamani, and L. Wu CD4 Coexpression Regulates DC-SIGN-Mediated Transmission of Human Immunodeficiency Virus Type 1 J. Virol., March 1, 2007; 81(5): 2497 - 2507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. U. Cameron, A. J. Handley, D. C. Baylis, A. E. Solomon, N. Bernard, D. F. J. Purcell, and S. R. Lewin Preferential Infection of Dendritic Cells during Human Immunodeficiency Virus Type 1 Infection of Blood Leukocytes J. Virol., March 1, 2007; 81(5): 2297 - 2306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Boggiano, N. Manel, and D. R. Littman Dendritic Cell-Mediated trans-Enhancement of Human Immunodeficiency Virus Type 1 Infectivity Is Independent of DC-SIGN J. Virol., March 1, 2007; 81(5): 2519 - 2523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gijzen, P. J. Tacken, A. Zimmerman, B. Joosten, I. J. M. de Vries, C. G. Figdor, and R. Torensma Relevance of DC-SIGN in DC-induced T cell proliferation J. Leukoc. Biol., March 1, 2007; 81(3): 729 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. van Montfort, A. A. Nabatov, T. B. H. Geijtenbeek, G. Pollakis, and W. A. Paxton Efficient Capture of Antibody Neutralized HIV-1 by Cells Expressing DC-SIGN and Transfer to CD4+ T Lymphocytes J. Immunol., March 1, 2007; 178(5): 3177 - 3185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bozzacco, C. Trumpfheller, F. P. Siegal, S. Mehandru, M. Markowitz, M. Carrington, M. C. Nussenzweig, A. G. Piperno, and R. M. Steinman DEC-205 receptor on dendritic cells mediates presentation of HIV gag protein to CD8+ T cells in a spectrum of human MHC I haplotypes PNAS, January 23, 2007; 104(4): 1289 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lissandrini, W. Vermi, M. Vezzalini, S. Sozzani, F. Facchetti, G. Bellone, A. Mafficini, F. Gentili, M. G. Ennas, C. Tecchio, et al. Receptor-type protein tyrosine phosphatase gamma (PTP{gamma}), a new identifier for myeloid dendritic cells and specialized macrophages Blood, December 15, 2006; 108(13): 4223 - 4231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Davis, L. M. Mattei, H.-Y. Nguyen, C. Ansarah-Sobrinho, R. W. Doms, and T. C. Pierson The Location of Asparagine-linked Glycans on West Nile Virions Controls Their Interactions with CD209 (Dendritic Cell-specific ICAM-3 Grabbing Nonintegrin) J. Biol. Chem., December 1, 2006; 281(48): 37183 - 37194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chaipan, E. J. Soilleux, P. Simpson, H. Hofmann, T. Gramberg, A. Marzi, M. Geier, E. A. Stewart, J. Eisemann, A. Steinkasserer, et al. DC-SIGN and CLEC-2 Mediate Human Immunodeficiency Virus Type 1 Capture by Platelets. J. Virol., September 1, 2006; 80(18): 8951 - 8960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Caminschi, A. J Corbett, C. Zahra, M. Lahoud, K. M Lucas, M. Sofi, D. Vremec, T. Gramberg, S. Pohlmann, J. Curtis, et al. Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN Int. Immunol., May 1, 2006; 18(5): 741 - 753. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
