The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Related articles in The JI
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xie, H.
Right arrow Articles by Sun, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xie, H.
Right arrow Articles by Sun, Z.
The Journal of Immunology, 2005, 175: 3800-3809.
Copyright © 2005 by The American Association of Immunologists

ROR{gamma}t Recruits Steroid Receptor Coactivators to Ensure Thymocyte Survival

Huimin Xie, Maureen S. Sadim1 and Zuoming Sun2

Department of Microbiology and Immunology, College of Medicine, University of Illinois, Chicago, IL 60612

Thymocytes undergo apoptosis unless a functional TCR is assembled. Steroid receptor coactivators (SRCs) regulate nuclear receptor-mediated transcription by associated histone acetyltransferase activity. However, it has been a challenge to demonstrate the in vivo function of SRCs due to the overlapping functions among different members of SRCs. In this study, we show that recruitment of SRCs is required for thymic-specific retinoic acid-related orphan receptor {gamma} (ROR{gamma})t-regulated thymocyte survival in vivo. An activation function 2 domain, identified at the carboxyl terminus of ROR{gamma}t, is responsible for recruiting SRCs. A mutation in the activation function domain (Y479F) of ROR{gamma}t disrupted the interaction with SRCs and abolished ROR{gamma}t-mediated trans-activation but not its ability to inhibit transcription. Transgenes encoding the wild-type ROR{gamma}t, but not the mutant, restored thymocyte survival in ROR{gamma} null mice. Our results thus clearly demonstrate that ROR{gamma}t recruits SRCs to impose a gene expression pattern required to expand the life span of thymocytes in vivo, which increases the opportunities for assembling a functional TCR.


Related articles in The JI:

IN THIS ISSUE

The JI 2005 175: 3457-3458. [Full Text]  



This article has been cited by other articles:


Home page
 J. Immunol.Home page
S. Manicassamy, D. Yin, Z. Zhang, L. L. Molinero, M.-L. Alegre, and Z. Sun
A Critical Role for Protein Kinase C-{theta}-Mediated T Cell Survival in Cardiac Allograft Rejection
J. Immunol., July 1, 2008; 181(1): 513 - 520.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Manicassamy, S. Gupta, Z. Huang, J. D. Molkentin, W. Shang, and Z. Sun
Requirement of Calcineurin A for the Survival of Naive T Cells
J. Immunol., January 1, 2008; 180(1): 106 - 112.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
S. Raichur, P. Lau, B. Staels, and G. E O Muscat
Retinoid-related orphan receptor {gamma} regulates several genes that control metabolism in skeletal muscle cells: links to modulation of reactive oxygen species production
J. Mol. Endocrinol., July 1, 2007; 39(1): 29 - 44.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M.-Y. Kim, F. M. McConnell, F. M. C. Gaspal, A. White, S. H. Glanville, V. Bekiaris, L. S. K. Walker, J. Caamano, E. Jenkinson, G. Anderson, et al.
Function of CD4+CD3- cells in relation to B- and T-zone stroma in spleen
Blood, February 15, 2007; 109(4): 1602 - 1610.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2005 by The American Association of Immunologists, Inc. All rights reserved.