HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Iyoda, T. Articles by Kobayashi, Y. Search for Related Content PubMed PubMed Citation Articles by Iyoda, T. Articles by Kobayashi, Y.

Neutrophils Accelerate Macrophage-Mediated Digestion of Apoptotic Cells In Vivo as Well as In Vitro¹

Takuya Iyoda^*, Kisaburo Nagata^*, Makoto Akashi and Yoshiro Kobayashi^2,*

^* Division of Molecular Medicine, Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Japan; and National Institute of Radiological Science, Chiba, Japan

It is generally believed that the clearance of apoptotic cells does not lead to inflammation. In contrast, we previously found that injection of apoptotic cells into the peritoneal cavity induced the expression of an inflammatory chemokine, MIP-2, and infiltration of neutrophils, and that anti-MIP-2 Abs suppressed the infiltration significantly. Because our previous study showed that whole-body x-irradiation caused neutrophil infiltration into the thymus along with T cell apoptosis, we examined the role of neutrophils in apoptotic cell clearance. Neutrophil infiltration reached a peak 12 h after irradiation with 1 Gy of x-rays. Immunohistological analysis revealed that apoptotic cells disappeared dramatically from 10.5 to 12 h after x-irradiation. As neutrophils moved from an inner area of the cortex to the periphery, apoptotic cells disappeared concomitantly. Either anti-MIP-2 or anti-CXCR2 Abs suppressed neutrophil infiltration significantly, and the suppression of neutrophil infiltration by anti-MIP-2 Abs delayed the disappearance of apoptotic cells. Moreover, macrophage-mediated digestion of apoptotic thymocytes was accelerated in vitro on coculturing with neutrophils, even if neutrophils were separated from macrophages. These results suggest that neutrophils are recruited to the thymus mainly by MIP-2 after whole-body x-irradiation and that such neutrophils may not induce inflammation but rather accelerate complete digestion of apoptotic cells by macrophages.

This article has been cited by other articles:

				T. Shibata, K. Nagata, and Y. Kobayashi Cutting Edge: A Critical Role of Nitrogen Oxide in Preventing Inflammation upon Apoptotic Cell Clearance J. Immunol., September 15, 2007; 179(6): 3407 - 3411. [Abstract] [Full Text] [PDF]

				Y. Hashimoto, T. Moki, T. Takizawa, A. Shiratsuchi, and Y. Nakanishi Evidence for Phagocytosis of Influenza Virus-Infected, Apoptotic Cells by Neutrophils and Macrophages in Mice J. Immunol., February 15, 2007; 178(4): 2448 - 2457. [Abstract] [Full Text] [PDF]

				Y. Yamaguchi, K. Takahashi, B. Z. Zmudzka, A. Kornhauser, S. A. Miller, T. Tadokoro, W. Berens, J. Z. Beer, and V. J. Hearing Human skin responses to UV radiation: pigment in the upper epidermis protects against DNA damage in the lower epidermis and facilitates apoptosis FASEB J, July 1, 2006; 20(9): 1486 - 1488. [Abstract] [Full Text] [PDF]