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Dichotomal Role of TNF in Experimental Pulmonary Edema Reabsorption

Clemens Braun^*, Jürg Hamacher^*, Denis R. Morel, Albrecht Wendel^1,* and Rudolf Lucas^1,*,

^* Department of Biochemical Pharmacology, University of Konstanz, Konstanz, Germany; Department of Anesthesiological Investigations, University Medical Center, Geneva, Switzerland; and Department of Pharmaceutical and Medical Biotechnology, University of Applied Sciences, Krems, Austria

Distinct from its receptor binding sites, TNF carries a lectin-like domain, situated at the tip of the molecule, which specifically binds oligosaccharides, such as N,N'-diacetylchitobiose. In view of the apparently conflicting data concerning TNF actions in pulmonary edema, we investigated the contribution of, on the one hand, the receptor binding sites and, in contrast, the lectin-like domain of the cytokine on pulmonary fluid reabsorption in in situ and in vivo flooded rat lungs. Receptor binding sites were blocked with the human soluble TNFR type 1 construct (sTNFR1), whereas the lectin-like domain was blunted with the oligosaccharide N,N'-diacetylchitobiose. We observed that in situ, TNF failed to stimulate alveolar liquid clearance, but did so together with the sTNFR1, and this activity was neutralized by N,N'-diacetylchitobiose. In vivo TNF inhibited liquid clearance, but activated it when complexed with the sTNFR1. A TNF-derived peptide mimic of the lectin-like domain activated fluid reabsorption in flooded lungs, and this activity was blunted by cotreatment with TNF. Our results thus indicate that in these models the receptor binding sites of TNF inhibit, whereas its lectin-like domain activates, edema reabsorption.

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