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A Helminth Glycan Induces APC Maturation via Alternative NF-B Activation Independent of IB Degradation¹

Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115

Activation of APCs via TLRs leads to activation of NF-B, a key transcription factor in cells of the immune system most often associated with induction of Th1-type and proinflammatory responses. The neoglycoconjugate lacto-N-fucopentaose III (12-25 molecules)-dextran (LNFPIII-Dex) activates dendritic cells (DCs) via TLR4, as does LPS. However, unlike LPS, LNFPIII-Dex-activated cells induce Th2-type CD4⁺ T cell responses. This observation led us to ask whether LNFPIII-activated APCs were differentially activating NF-B, and if so, could this partly account for how DCs mature in response to these two different pathogen-associated molecular patterns (PAMPs). In this study, we show that LNFPIII-Dex stimulation of APCs induces rapid, but transient NF-B translocation and activity in the nucleus, in comparison with the persistent activation induced by LPS. We then demonstrate that transient vs persistent NF-B activation has important implications in the development of the APC phenotype, showing that the second wave of NF-B translocation in response to LPS is required for production of the proinflammatory mediator NO. In contrast to LPS, LNFPIII-stimulated APCs that only transiently activate NF-B do not induce degradation of the known IB family members or production of NO. However, cells stimulated with LNFPIII rapidly accumulate p50, suggesting that an alternative p105 degradation-dependent mechanism is primarily responsible for NF-B activation downstream of LNFPIII. Finally, we show that while NF-B translocation in LNFPIII-stimulated APCs is transient, it is required for the development of the DC 2 phenotype, confirming a crucial and multifaceted role for NF-B in innate immune responses.

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